Search Results (2)

Based on previous researches, we further investigated the multi-silique trait in rapeseed (Brassica napus L.) line zws-ms. In this study, we used a relatively comprehensive list of flowering related genes in rapeseed and compared them between zws-ms and its near-isogenic line (NIL) zws-217. Genes were studied on genome, transcriptome and proteome levels and then we focused on genes with non-synonymous single nucleotide polymorphism (SNP) or frame-shift insertion-deletion (InDel), finding some genes on the list which changes their sequences. Then, combined with their annotation and the information of their orthologs, certain genes such as BnaA09g05900D, ortholog of AGAMOUS-LIKE 42 (AGL42), which encodes an MADS-box protein, were assumed as probably responsible for the multi-silique trait. Also, we analyzed the Differentially Accumulated Proteins (DAPs) between zws-ms and zws-217, revealing some genes involved in homologous recombination and mismatch repair pathways. Since the development of flowers/siliques is crucial to crops and it influences the yield of rapeseed, this study paved a way to deeply understand the mechanism of the multi-pistil flower formation, which may facilitate researches on rapeseed production in future. Full article

The effect of genotype, environment (year, location) and their interaction on seed yield and important breeding traits of 25 genotypes of winter oilseed rape were investigated under field conditions in Greater Poland. Multi-environmental field experiments were conducted in a randomized block design with four replications during three growing seasons in two locations. Five traits, such as the beginning of flowering, seed yield and its structure, the length of siliques, the number of seeds per silique and the weight of 1000 seeds were recorded. The tested Brassica genotypes showed significant differences in terms of yield and other investigated traits across harvesting years and growing locations. Analysis of variance indicated that the main effects of genotypes, locations and years as well as all interactions were significant for all traits of study. The correlation coefficient between the investigated traits displayed strong negative relationships between seed yield and the beginning of flowering (except E2). The use of multivariate statistical methods in this study allowed for the simultaneous characterization of 25 tested genotypes in terms of several traits. Visualization of the experimental results and finally the distribution of Brassica genotypes in space of two first canonical variates showed a variation between the cultivars, double low, resynthesized and lines with changed fatty acid content in terms of yield and its components, as well as the beginning of flowering. Full article