Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

remove_circle_outline

Search Results (1)

Search Parameters:
Keywords = multi-channel Cellytics

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
15 pages, 3020 KB  
Article
Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation
by Hojin Cheon, Samir Kumar, Inha Lee, Sanghoon Shin, Hyeji Jang, Young-Sun Lee, Myung-Hyun Nam, Hyun Sik Jun and Sungkyu Seo
Biosensors 2025, 15(3), 143; https://doi.org/10.3390/bios15030143 - 24 Feb 2025
Cited by 2 | Viewed by 1432
Abstract
Morphological changes in leukocytes are valuable markers for diseases and immune responses. In our earlier work, we presented Cellytics, a device that uses lens-free shadow imaging technology (LSIT) to monitor natural killer cell activity. Here, we present an improved Cellytics system that has [...] Read more.
Morphological changes in leukocytes are valuable markers for diseases and immune responses. In our earlier work, we presented Cellytics, a device that uses lens-free shadow imaging technology (LSIT) to monitor natural killer cell activity. Here, we present an improved Cellytics system that has been upgraded to a four-channel configuration to achieve higher throughput while maintaining robust reproducibility for rapid and cost-effective leukocyte analysis. The performance of this multi-channel Cellytics system was improved through refinements to the micro-pinhole chip. Etched pinholes provided better image resolution and clarity compared to drilled pinholes. To stimulate leukocytes, we used an activation stimulator cocktail (ASC) and quantified the resulting morphological changes using shadow-based metrics, including peak-to-peak distance (PPD) and maxima-to-minima standard deviation (MMD-SD). In addition, we developed a new leukocyte activation parameter (LAP) to specifically assess these activation-induced morphological changes. After ASC stimulation, leukocytes showed significantly increased PPD and LAP values and decreased MMD-SD compared to non-activated leukocytes. These results are consistent with the results of the flow cytometric analysis. These results emphasize the potential of Cellytics for the rapid and accurate assessment of leukocyte activation and provide a valuable tool for both clinical diagnostics and basic immunological research. Full article
Show Figures

Figure 1

Back to TopTop