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Keywords = microfluidic impedance and imaging flow cytometry

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14 pages, 2630 KiB  
Review
Developments of Conventional and Microfluidic Flow Cytometry Enabling High-Throughput Characterization of Single Cells
by Minruihong Wang, Hongyan Liang, Xiao Chen, Deyong Chen, Junbo Wang, Yuan Zhang and Jian Chen
Biosensors 2022, 12(7), 443; https://doi.org/10.3390/bios12070443 - 23 Jun 2022
Cited by 22 | Viewed by 4398
Abstract
This article first reviews scientific meanings of single-cell analysis by highlighting two key scientific problems: landscape reconstruction of cellular identities during dynamic immune processes and mechanisms of tumor origin and evolution. Secondly, the article reviews clinical demands of single-cell analysis, which are complete [...] Read more.
This article first reviews scientific meanings of single-cell analysis by highlighting two key scientific problems: landscape reconstruction of cellular identities during dynamic immune processes and mechanisms of tumor origin and evolution. Secondly, the article reviews clinical demands of single-cell analysis, which are complete blood counting enabled by optoelectronic flow cytometry and diagnosis of hematologic malignancies enabled by multicolor fluorescent flow cytometry. Then, this article focuses on the developments of optoelectronic flow cytometry for the complete blood counting by comparing conventional counterparts of hematology analyzers (e.g., DxH 900 of Beckman Coulter, XN-1000 of Sysmex, ADVIA 2120i of Siemens, and CELL-DYN Ruby of Abbott) and microfluidic counterparts (e.g., microfluidic impedance and imaging flow cytometry). Future directions of optoelectronic flow cytometry are indicated where intrinsic rather than dependent biophysical parameters of blood cells must be measured, and they can replace blood smears as the gold standard of blood analysis in the near future. Full article
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