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Keywords = microexplants

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15 pages, 1693 KB  
Article
3D In Vitro Platform for Cell and Explant Culture in Liquid-like Solids
by Duy T. Nguyen, Jack E. Famiglietti, Ryan A. Smolchek, Zadia Dupee, Nickolas Diodati, Diego I. Pedro, Juan M. Urueña, Matthew A. Schaller and W. Gregory Sawyer
Cells 2022, 11(6), 967; https://doi.org/10.3390/cells11060967 - 11 Mar 2022
Cited by 12 | Viewed by 7133
Abstract
Existing 3D cell models and technologies have offered tools to elevate cell culture to a more physiologically relevant dimension. One mechanism to maintain cells cultured in 3D is by means of perfusion. However, existing perfusion technologies for cell culture require complex electronic components, [...] Read more.
Existing 3D cell models and technologies have offered tools to elevate cell culture to a more physiologically relevant dimension. One mechanism to maintain cells cultured in 3D is by means of perfusion. However, existing perfusion technologies for cell culture require complex electronic components, intricate tubing networks, or specific laboratory protocols for each application. We have developed a cell culture platform that simply employs a pump-free suction device to enable controlled perfusion of cell culture media through a bed of granular microgels and removal of cell-secreted metabolic waste. We demonstrated the versatile application of the platform by culturing single cells and keeping tissue microexplants viable for an extended period. The human cardiomyocyte AC16 cell line cultured in our platform revealed rapid cellular spheroid formation after 48 h and ~90% viability by day 7. Notably, we were able to culture gut microexplants for more than 2 weeks as demonstrated by immunofluorescent viability assay and prolonged contractility. Full article
(This article belongs to the Special Issue 10th Anniversary of Cells—Advances in Cell Microenvironment)
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