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Keywords = low power STED

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23 pages, 5717 KB  
Review
Recent Advances in Fluorescent Nanoparticles for Stimulated Emission Depletion Imaging
by Liqing Qi, Songlin Liu, Jiantao Ping, Xingxing Yao, Long Chen, Dawei Yang, Yijun Liu, Chenjing Wang, Yating Xiao, Lubin Qi, Yifei Jiang and Xiaohong Fang
Biosensors 2024, 14(7), 314; https://doi.org/10.3390/bios14070314 - 21 Jun 2024
Cited by 10 | Viewed by 3959
Abstract
Stimulated emission depletion (STED) microscopy, as a popular super-resolution imaging technique, has been widely used in bio-structure analysis and resolving the dynamics of biological processes beyond the diffraction limit. The performance of STED critically depends on the optical properties of the fluorescent probes. [...] Read more.
Stimulated emission depletion (STED) microscopy, as a popular super-resolution imaging technique, has been widely used in bio-structure analysis and resolving the dynamics of biological processes beyond the diffraction limit. The performance of STED critically depends on the optical properties of the fluorescent probes. Ideally, the probe should process high brightness and good photostability, and exhibit a sensitive response to the depletion beam. Organic dyes and fluorescent proteins, as the most widely used STED probes, suffer from low brightness and exhibit rapid photobleaching under a high excitation power. Recently, luminescent nanoparticles (NPs) have emerged as promising fluorescent probes in biological imaging due to their high brightness and good photostability. STED imaging using various kinds of NPs, including quantum dots, polymer dots, carbon dots, aggregation-induced emission dots, etc., has been demonstrated. This review will comprehensively review recent advances in fluorescent NP-based STED probes, discuss their advantages and pitfalls, and outline the directions for future development. Full article
(This article belongs to the Special Issue Activatable Probes for Biosensing, Imaging, and Photomedicine)
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12 pages, 4811 KB  
Article
Ultralow Laser Power Three-Dimensional Superresolution Microscopy Based on Digitally Enhanced STED
by Xiaochun Shen, Luwei Wang, Wei Li, He Wang, Hanqiu Zhou, Yinru Zhu, Wei Yan and Junle Qu
Biosensors 2022, 12(7), 539; https://doi.org/10.3390/bios12070539 - 20 Jul 2022
Cited by 4 | Viewed by 2987
Abstract
The resolution of optical microscopes is limited by the optical diffraction limit; in particular, the axial resolution is much lower than the lateral resolution, which hinders the clear distinction of the three-dimensional (3D) structure of cells. Although stimulated emission depletion (STED) superresolution microscopy [...] Read more.
The resolution of optical microscopes is limited by the optical diffraction limit; in particular, the axial resolution is much lower than the lateral resolution, which hinders the clear distinction of the three-dimensional (3D) structure of cells. Although stimulated emission depletion (STED) superresolution microscopy can break through the optical diffraction limit to achieve 3D superresolution imaging, traditional 3D STED requires high depletion laser power to acquire high-resolution images, which can cause irreversible light damage to biological samples and probes. Therefore, we developed an ultralow laser power 3D STED superresolution imaging method. On the basis of this method, we obtained lateral and axial resolutions of 71 nm and 144 nm, respectively, in fixed cells with 0.65 mW depletion laser power. This method will have broad application prospects in 3D superresolution imaging of living cells. Full article
(This article belongs to the Special Issue Optical Imaging and Biophotonic Sensors (OIBS))
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13 pages, 2921 KB  
Article
Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging
by Jia Zhang, Xinwei Gao, Luwei Wang, Yong Guo, Yinru Zhu, Zhigang Yang, Wei Yan and Junle Qu
Biosensors 2021, 11(9), 330; https://doi.org/10.3390/bios11090330 - 10 Sep 2021
Cited by 10 | Viewed by 4388
Abstract
Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a high [...] Read more.
Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a high depletion power is required to obtain a high resolution. However, a high laser power can induce severe phototoxicity and photobleaching, which limits the applications for live cell imaging, especially in two-color STED super-resolution imaging. Therefore, we developed a low-power two-color STED super-resolution microscope with a single supercontinuum white-light laser. Using this system, we achieved low-power two-color super-resolution imaging based on digital enhancement technology. Lateral resolutions of 109 and 78 nm were obtained for mitochondria and microtubules in live cells, respectively, with 0.8 mW depletion power. These results highlight the great potential of the novel digitally enhanced two-color STED microscopy for long-term dynamic imaging of live cells. Full article
(This article belongs to the Special Issue Feature Issue of Optical and Photonic Biosensors Section)
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