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Keywords = locally enhanced electric field treatment (LEEFT)

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17 pages, 3876 KiB  
Article
Locally Enhanced Electric Field Treatment of E. coli: TEM, FT-IR and Raman Spectrometry Study
by Irina Saraeva, Dmitry Zayarny, Eteri Tolordava, Alena Nastulyavichus, Roman Khmelnitsky, Dmitry Khmelenin, Svetlana Shelygina and Sergey Kudryashov
Chemosensors 2023, 11(7), 361; https://doi.org/10.3390/chemosensors11070361 - 27 Jun 2023
Cited by 9 | Viewed by 2309
Abstract
In this paper, we report the study of the low-voltage locally enhanced electric field treatment (LEEFT) of E. coli bacteria via TEM analysis and FT-IR and Raman spectrometry. The formation of pores was confirmed by TEM, which revealed the membrane rupture along with [...] Read more.
In this paper, we report the study of the low-voltage locally enhanced electric field treatment (LEEFT) of E. coli bacteria via TEM analysis and FT-IR and Raman spectrometry. The formation of pores was confirmed by TEM, which revealed the membrane rupture along with the formation of precipitates in the membrane and the cell volume, and by photoluminescence spectra of propidium iodide dye. LEEFT results in the alternation of DNA and RNA structure, indicated by the change in its α-helical and β-helical forms, decrease and narrowing of the nucleic acids-related IR and Raman peaks. Membrane damage is illustrated by the ambiguous character after low-voltage exposure: several membrane fatty acids’ peaks are broadening, while the others narrow, which indicates the overall change of the molecular bonds in their moiety, and may result from the rigidification during the shrinkage of the inner membrane and the increase in fluidity of the outer membrane. The corresponding fingerprints of cytochrome c and bo, located in the membrane or in the periplasm, on Raman spectra, reflect the arising changes in their structure and moiety. The observed changes were partially confirmed by TEM images, which indicate the dense precipitates’ formation in the cell volume and membrane, as well as the cytoplasmic membrane shrinkage away from the outer membrane. Full article
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16 pages, 24300 KiB  
Article
The Bacterial Disinfection of Water Using a Galloping Piezoelectric Wind Energy Harvester
by Prakash Poudel, Saurav Sharma, Mohamed Nainar Mohamed Ansari, Pushpendra Kumar, Sobhy M. Ibrahim, Rahul Vaish, Rajeev Kumar and Paramanandam Thomas
Energies 2022, 15(17), 6133; https://doi.org/10.3390/en15176133 - 24 Aug 2022
Cited by 2 | Viewed by 2110
Abstract
In this study, a method for the bacterial disinfection of drinking water in the water storage systems based on the electric potential generated from a piezoelectric wind energy harvester is presented. First, an efficient galloping piezoelectric wind energy harvester is designed by adding [...] Read more.
In this study, a method for the bacterial disinfection of drinking water in the water storage systems based on the electric potential generated from a piezoelectric wind energy harvester is presented. First, an efficient galloping piezoelectric wind energy harvester is designed by adding curve- shaped attachments to the bluff body of the harvester. The simulated output voltage of the harvester is validated by performing different sets of experiments on an open environment. Later, the output voltage of the harvester is enhanced, using copper oxide nanowires (CuONWs) grown perpendicular to the surface of the center copper wire. The enhanced electric field is able to disinfect the bacterial water in a 25 min time period. The bacterial removal log efficiency of 2.33 is obtained with a supplied rms voltage of 0.1 V from the harvester. The findings of this study will help to provide alternate means to water treatment that are efficient, reliable, and also free from disinfection by-products. Full article
(This article belongs to the Section B: Energy and Environment)
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