Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

Search Results (1)

Search Parameters:
Keywords = klosneuvirinae

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
10 pages, 7364 KiB  
Article
Diversity of Amoeba-Associated Giant Viruses Isolated in Algeria
by Hadjer Boudjemaa, Julien Andreani, Idir Bitam and Bernard La Scola
Diversity 2020, 12(6), 215; https://doi.org/10.3390/d12060215 - 29 May 2020
Cited by 6 | Viewed by 3852
Abstract
The discovery of several giant amoeba viruses has opened up a novel area in the field of virology. Despite this, knowledge about ecology of these viruses remains patchy. In this study, we aimed to characterize the diversity of giant viruses in Algeria by [...] Read more.
The discovery of several giant amoeba viruses has opened up a novel area in the field of virology. Despite this, knowledge about ecology of these viruses remains patchy. In this study, we aimed to characterize the diversity of giant viruses in Algeria by inoculating 64 environmental samples on various amoeba strains. After isolation by co-culture with nine amoeba supports, flow cytometry and electron microscopy were used to putatively identify viruses. Definitive identification was performed by PCR and sequencing. Mimiviruses, marseilleviruses, faustoviruses and cedratviruses were the main viruses isolated in this study. Moreover, a new virus, which we named fadolivirus, was also isolated and was found to belong to the recent metagenomic descriptions of Klosneuvirinae. Despite the use of 9 amoeba supports for co-culture, most of the isolates were obtained from two amoebas: Acanthamoeba castellanii Neff and Vermamoeba vermiformis CDC 19. Finally, the viruses most frequently isolated were marseilleviruses (55.5%) and Mimiviruses (22.2%). This work shows that the isolation of viruses previously detected by metagenomic analyses can be tedious, but possible. Full article
(This article belongs to the Special Issue Giant Virus Biology and Biodiversity)
Show Figures

Figure 1

Back to TopTop