Search Results (6)

As a novel microfabrication method, electrochemical discharge machining has remarkable effects on the forming and processing of brittle and hard materials and non-conductive materials, but little research has been done on the electrochemical discharge mode in the jet state. To fulfil the potential of this technology, innovative research on the discharge characteristics and mechanism of electrochemical discharge machining in the jet mask is proposed. A high-speed camera observation experiment was set up to record the process of the jet flow column discharge formation and penetration. Changes in the electric field of the electrolytic jet channel were analysed by simulation software, and the morphology of the machined micro-pits was observed using a microscope. A mathematical derivation of the dielectric electric field in the gas–liquid two-phase jet column reveals the mechanism of discharge channel formation in the jet state. The experiments show that when the processing voltage is 400 V, a stable continuous spark appears, realizing the unique characteristics of a large-gap long-distance discharge and a flat small circle-shaped discharge mark produced at the bottom of the crater. The actual field strength within the bubble of this model obtained by mathematical derivation is approximately 61.5 kV/cm greater than the critical field strength for air bubble breakdown in the standard state, where bubble breakdown occurs in the discharge. Full article

In order to study the ignition process and response characteristics of cast polymer-bonded explosives (PBX) under the action of friction, HMX-based cast PBX explosives were used to carry out friction ignition experiments at a 90° swing angle and obtain the critical ignition loading pressure was 3.7 MPa. Combined with the morphology characterization results of HMX-based cast PBX, the friction temperature rise process was numerically simulated at the macro and micro scale, and the ignition characteristics were judged. The accuracy of the numerical simulation results was ensured based on the experiment. Based on the thermal–mechanical coupling algorithm, the mechanical–thermal response of HMX-based cast PBX tablet under friction was analyzed from the macro scale. The results show that the maximum temperature rise is 55 °C, and the temperature rise of the whole tablet is not enough to ignite the explosive. Based on the random circle and morphology characterization results of tablet, the mesoscopic model of HMX-based cast PBX was constructed, and the microcrack friction formed after interface debonding was introduced into the model. The temperature rise process at the micro scale shows that HMX crystal particles can be ignited at a temperature of 619 K under 4 MPa hydraulic pressure loaded by friction sensitivity instrument. The main reason for friction ignition of HMX-based cast PBX is the friction hot spot generated by microcracks formed after interface damage of the tablet mesoscopic model, and the external friction heat between cast PBX tablet and sliding column has little effect on ignition. External friction affects the ignition of HMX-based cast PBX by influencing the formation of internal cracks and the stress at microcracks. Full article

Background: The Bcl2-associated athanogene4 (BAG4/SODD) protein could be identified as a tumor marker for several malignancies and plays a major role in the occurrence, development, and drug resistance of tumors. However, the role of Silencer of death domains (SODD) in lung carcinogenesis is still elusive. Objective: To illuminate the effect of SODD on the proliferation, migration, invasion, and apoptosis of lung cancer cells and tumor growth in vivo and explore the corresponding mechanism. Methods: The expression of SODD in tumor and normal tissues was determined and compared via western blot. SODD gene knockout lung cancer cells (H1299 cells) were established through a CRISPR/Cas9 gene deleting system, and a transient SODD overexpression of H1299 cells was also constructed. Then, cell proliferation and invasion were assessed through colony formation and cell counting kit-8 assays, transwell migration assays, and wound healing assays. Cell drug sensitivity is also analyzed by Cell Counting Kit-8 assay. The flow cytometer was used to perform cell circle and apoptosis analysis. The interaction of SODD and RAF-1 was confirmed by co-immunoprecipitation, and the phosphorylated level of Phosphatidylinositol 3-kinase (PI3K), Serine/threonine-protein kinase (AKT), Rapidly accelerated fibrosarcoma (RAF)-1,and extracellular signal regulated kinase (ERK) in cells was examined by western blot to evaluate the activation of PI3K/PDK1/AKT and RAF/MEK/ERK pathways. In vivo, Xenograft tumor assay of SODD knockout H1299 cells was used to evaluate further the role of SODD on the proliferation of H1299 cells. Results: SODD binds to RAF-1 and is over-expressed in lung tissues, and promotes the proliferation, migration, invasion, and drug sensitivity of H1299 cells. The reduced cells in the S phase and increased cells arrested in the G2/M phase were found in SODD knockout H1299 cells, and more cells got apoptosis. The expression of 3-phosphoinositide-dependent protein kinase 1(PDK1) protein in SODD knockout H1299 cells decreases distinctively, and the phosphorylated level of AKT, RAF-1, and ERK-1 kinase in SODD knockout H1299 cells is also less than that in normal H1299 cells. In contrast, SODD overexpression significantly increases the phosphorylation of AKT. In vivo, SODD promotes the tumorigenicity of H1299 cells in nude mice. Conclusions: SODD is overexpressed in lung tissues and plays a considerable role in the development and progression of lung cancer by regulating the PI3K/PDK1/AKT and RAF/MEK/ERK pathways. Full article

A novel Co-free non-equiatomic Ni₂FeCr medium-entropy alloy (MEA) was designed, and the Hall–Petch strengthening by grain boundaries and annealing twin boundaries was investigated. For this purpose, the alloy was prepared by cold rolling and recrystallization at 873–1323 K for 40 min–6 h. Annealing at different temperatures revealed that Ni₂CrFe alloy forms a stable face-centered cubic (FCC) solid solution. Mean grain sizes (excluding annealing twin boundaries) and mean crystallite sizes (including both grain and annealing twin boundaries) were determined using the linear intercept method and the equivalent circle diameter in electron back-scattered diffraction (EBSD) soft. Tensile tests at 293 K indicated that the Hall-Petch slopes of grain sizes and crystallite sizes are 673 and 544 MPa μm^1/2, respectively, and this contribution was then subtracted from the overall strength to calculate the intrinsic uniaxial lattice strength (90 MPa). Additionally, tensile tests, performed between 293 K and 873 K, revealed that the Ni₂CrFe MEA has a stronger resistance to softening at high temperatures. Transmission electron microscopy of deformed specimens revealed the formation of dislocation pile-ups at annealing twin boundaries, indicating that it is also an obstacle to dislocation slip. Furthermore, the thickening of the annealing twin boundary after deformation was observed and illustrated by the interaction between different dislocations and annealing twin boundaries. Full article

In many circles, including in Poland, lignite is still viewed as a cheap source of energy, which is only possible if the external costs associated with mining and burning coal are not taken into account. In Poland, this is reflected in plans to open new Złoczew opencast lignite mines. In previous studies, the analysis of external costs has focused on the external costs of coal combustion and related pollutant emissions. This paper focuses on the extraction phase. The aim of the work here described was to estimate the external costs that agriculture may incur due to the formation of a depression funnel for the projected lignite mine in the Złoczew deposit. This paper discusses factors causing uncertainty in calculated estimates of external costs in agriculture, and characterizes the Bełchatów and Złoczew opencast mines. In the paper, a methodology for calculating external costs in livestock production is then proposed. In the next part of the study, the decrease in cereal and potato yields and in the number of cattle and pigs in the area of the cone of depression of the Bełchatów opencast mine, which has been in operation for 40 years, were estimated. The estimates obtained formed the basis for estimating external costs for the planned Złoczew lignite opencast. The analyses showed high external costs for plant production and much lower for animal production. The inclusion of the estimated external costs of 12.2 € × kWh⁻¹ in the costs of electricity production will significantly worsen the profitability of launching this opencast. The paper discusses factors causing uncertainty in calculated estimates of external costs in agriculture, and characterizes the Bełchatów and Złoczew opencast mines. The discussion also shows that the level of losses incurred in crop production due to opencast coal mining is similar to the losses incurred in crop production in extremely dry years. Full article

For a given positive integer k, the k-circle formation problem asks a set of autonomous, asynchronous robots to form disjoint circles having k robots each at distinct locations, centered at a set of fixed points in the Euclidean plane. The robots are identical, anonymous, oblivious, and they operate in Look–Compute–Move cycles. This paper studies the k-circle formation problem and its relationship with the k-epf problem, a generalized version of the embedded pattern formation problem, which asks exactly k robots to reach and remain at each fixed point. First, the k-circle formation problem is studied in a setting where the robots have an agreement on the common direction and orientation of one of the axes. We have characterized all the configurations and the values of k, for which the k-circle formation problem is deterministically unsolvable in this setting. For the remaining configurations and the values of k, a deterministic distributed algorithm has been proposed, in order to solve the problem. It has been proved that for the initial configurations with distinct robot positions, if the k-circle formation problem is deterministically solvable then the k-epf problem is also deterministically solvable. It has been shown that by modifying the proposed algorithm, the k-epf problem can be solved deterministically. Full article