Search Results (2)

The isochore theory, which was proposed more than 40 years ago, depicts the mammalian genome as a mosaic of long, homogeneous regions that are characterized by their guanine and cytosine (GC) content. The human genome, for instance, was claimed to consist of five compositionally distinct isochore families. The isochore theory, in all its reincarnations, has been repeatedly falsified in the literature, yet isochore proponents have persistently resurrected it by either redefining isochores or by proposing alternative means of testing the theory. Here, I deal with the latest attempt to salvage this seemingly immortal zombie—a sequence segmentation method called isoSegmenter, which was claimed to “identify” isochores while at the same time disregarding the main characteristic attribute of isochores—compositional homogeneity. I used a series of controlled, randomly generated simulated sequences as a benchmark to study the performance of isoSegmenter. The main advantage of using simulated sequences is that, unlike real data, the exact start and stop point of any isochore or homogeneous compositional domain is known. Based on three key performance metrics—sensitivity, precision, and Jaccard similarity index—isoSegmenter was found to be vastly inferior to isoPlotter, a segmentation algorithm with no user input. Moreover, isoSegmenter identified isochores where none exist and failed to identify compositionally homogeneous sequences that were shorter than 100−200 kb. Will this zillionth refutation of “isochores” ensure a final and permanent entombment of the isochore theory? This author is not holding his breath. Full article

Floor covering samples of different thickness, pile height, pile design, materials, construction methods, and applied finishes were selected for electrostatic characterization with a standard plotter platform and a newly designed digital platform. There is an existing standard ISO 6356 in which the voltage generated by a human walking on the carpet is measured with human involvement under controlled conditions. A walking person performs the original test procedure to generate the electrostatic charge and manually calculates results. In contrast, the newly designed system does not require a person to calculate peaks and valleys for the generated electrostatic charges, which offers advantages in terms of accuracy, consistency, and reproducibility, and eliminates human error. The electronic platform is extended with an automated foot for a fully automated test, called “automatic mode”, that has a fixed capacitive and resistive circuit, in replace of human body resistance, and capacitance that varies from person to person and over time. The procedure includes both the old and new platforms, where the new platform is placed in a “human walking” mode to compare the two and validate the new device. Next, all the floor coverings are tested in automatic mode with the automated foot to compare and validate results. We conclude that the new testing device can fully characterize the electrostatic behavior of textile without the involvement of a human, which offers advantages in terms of accuracy, consistency, and reproducibility. Full article