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Keywords = halo-bridged complexes

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13 pages, 8261 KiB  
Article
Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers
by Katarina Molnarova and Petr Kozlík
Molecules 2020, 25(20), 4655; https://doi.org/10.3390/molecules25204655 - 13 Oct 2020
Cited by 35 | Viewed by 4893
Abstract
Protein glycosylation analysis is challenging due to the structural variety of complex conjugates. However, chromatographically separating glycans attached to tryptic peptides enables their site-specific characterization. For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary [...] Read more.
Protein glycosylation analysis is challenging due to the structural variety of complex conjugates. However, chromatographically separating glycans attached to tryptic peptides enables their site-specific characterization. For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers. Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins. The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention. The HALO® penta-HILIC column provided the best separation results, and the ZIC-HILIC column the worst. Moreover, we showed the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms. Therefore, HILIC is a useful tool for the comprehensive characterization of glycoproteins and their isomers. Full article
(This article belongs to the Special Issue Advances on Glycoconjugates – Chemistry and Chemical Biology)
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10 pages, 469 KiB  
Article
Bent Dinuclear Platinum(II) Halo-Bridged Carbonyl Complexes
by Daniela Belli Dell'Amico, Luca Labella and Fabio Marchetti
Molecules 2011, 16(7), 6082-6091; https://doi.org/10.3390/molecules16076082 - 20 Jul 2011
Cited by 1 | Viewed by 6135
Abstract
Crystals of trans-Pt2(μ-X)2X2(CO)2 (X = Br, I) have been grown and their molecular and crystalline structures have been solved by X-ray diffraction methods. In both cases the dinuclear molecules are bent, with a [...] Read more.
Crystals of trans-Pt2(μ-X)2X2(CO)2 (X = Br, I) have been grown and their molecular and crystalline structures have been solved by X-ray diffraction methods. In both cases the dinuclear molecules are bent, with a bending angle of 164.6° and 156.5° for the bromide and the iodide, respectively. While the structure of the bromo-derivative is reported here for the first time, a modification of trans-Pt2(μ-I)2I2(CO)2 with planar centrosymmetric molecules is known. This appears to be a rare case of a platinum(II) halo-bridged derivative structurally characterized in both bent and planar forms. Full article
(This article belongs to the Special Issue Organometallic Chemistry)
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