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Keywords = gnetumoside B

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13 pages, 21882 KiB  
Article
Two New Steroidal Saponins with Potential Anti-Inflammatory Effects from the Aerial Parts of Gnetum formosum Markgr.
by Ngo Van Hieu, Le Ba Vinh, Nguyen Viet Phong, Pham Van Cong, Nguyen Tien Dat, Nguyen Van Dan, Ngo Viet Duc, Hoang Minh Tao, Le Thi Tam, Le Tuan Anh, Nguyen Cao Cuong, Bui Huu Tai, Seo Young Yang and Hoang Le Tuan Anh
Plants 2024, 13(15), 2100; https://doi.org/10.3390/plants13152100 - 29 Jul 2024
Cited by 4 | Viewed by 1836
Abstract
Gnetum formosum Markgr., a member of the Gnetaceae family, is distributed in Vietnam. This plant remains a botanical enigma with an unexplored diversity of chemical constituents and pharmacological effects. In this study, two new steroidal saponins, namely gnetumosides A (1) and [...] Read more.
Gnetum formosum Markgr., a member of the Gnetaceae family, is distributed in Vietnam. This plant remains a botanical enigma with an unexplored diversity of chemical constituents and pharmacological effects. In this study, two new steroidal saponins, namely gnetumosides A (1) and B (2), were isolated from the aerial parts of G. formosum. Their chemical structures were elucidated using spectroscopic techniques, including high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and NMR, along with chemical hydrolysis and comparison with the reported literature. The potential anti-inflammatory effects of the isolated compounds were evaluated by measuring lipopolysaccharide-stimulated nitric oxide (NO) production in murine macrophage cells. Notably, compound 1 exhibited the most potent inhibitory activity (IC50 = 14.10 ± 0.75 µM), comparable to dexamethasone. Additionally, the mechanisms underlying the observed anti-inflammatory effects were investigated through molecular docking and molecular dynamics simulations on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins. This study is the first to investigate the chemical constituents and pharmacological effects of G. formosum. Full article
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