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Keywords = glycerol ethoxylate-co-propoxylate triol

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11 pages, 897 KiB  
Communication
Ethoxylate Polymer-Based 96-Well Screen for Protein Crystallization
by Ulrike Demmer, Olivier N. Lemaire, Mélissa Belhamri and Ulrich Ermler
Crystals 2023, 13(10), 1519; https://doi.org/10.3390/cryst13101519 - 19 Oct 2023
Cited by 1 | Viewed by 1747
Abstract
Crystallization is the limiting step in X-ray structure determination of biological macromolecules. As crystallization experiments can be largely automatized, the diversity of precipitant solutions is often the determinant factor to obtain crystals of high quality. Here, we introduce a 96-well screening kit of [...] Read more.
Crystallization is the limiting step in X-ray structure determination of biological macromolecules. As crystallization experiments can be largely automatized, the diversity of precipitant solutions is often the determinant factor to obtain crystals of high quality. Here, we introduce a 96-well screening kit of crystallization conditions, centered on three ethoxylate-based organic polymers as precipitants and various additional compounds to promote crystal formation. This crystallization screen was tested on various non-standard proteins from bacteria and archaea. Structure determination succeeded for seven out of thirteen targets based on crystals that frequently diffracted to a higher resolution than those obtained with commercially available screening kits. Crystallization hits were rarely similar among the three ethoxylate-based organic polymers and, in comparison, with already available crystallization screens. Hence, the presented crystallization screen is an efficient tool to complement other screens and increase the likelihood of growing crystals suitable for X-ray structure determination. Full article
(This article belongs to the Special Issue Macromolecular Crystallography: Progress and Prospects)
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