The use of α-glucosidase inhibitors is considered to be an effective strategy in the treatment of diabetes. Using a bioassay-guided fractionation technique, five
Bacillus stearothermophilus α-glucosidase inhibitors were isolated from the flowers of
Musa spp. (Baxijiao). Using NMR spectroscopy analysis they were identified
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The use of α-glucosidase inhibitors is considered to be an effective strategy in the treatment of diabetes. Using a bioassay-guided fractionation technique, five
Bacillus stearothermophilus α-glucosidase inhibitors were isolated from the flowers of
Musa spp. (Baxijiao). Using NMR spectroscopy analysis they were identified as vanillic acid (
1), ferulic acid (
2), β-sitosterol (
3), daucosterol (
4) and 9-(4′-hydroxyphenyl)-2-methoxyphenalen-1-one (
5). The half maximal inhibitory concentration (IC
50) values of compounds
1–
5 were 2004.58, 1258.35, 283.67, 247.35 and 3.86 mg/L, respectively. Compared to a known α-glucosidase inhibitor (acarbose, IC
50 = 999.31 mg/L), compounds
3,
4 and
5 showed a strong α-glucosidase inhibitory effect. A Lineweaver-Burk plot indicated that compound
5 is a mixed-competitive inhibitor, while compounds
3 and
4 are competitive inhibitors. The inhibition constants (
Ki) of compounds
3,
4 and
5 were 20.09, 2.34 and 4.40 mg/L, respectively. Taken together, these data show that the compounds
3,
4 and
5 are potent α-glucosidase inhibitors.
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