Search Results (7)

The nutrient availability of carbon (C), nitrogen (N), and phosphorus (P) has been decreasing due to a decline in the biological function of yellow soil, limiting potato yield (PY). Increasing biochar or organic fertilizer input is an effective way to improve soil microbiological fertility. However, indexes to regulate soil microbiological fertility using biochar and organic fertilizer individually or in combination and these indexes’ associations with PY remain unclear. In this study, four fertilization strategies were developed using the nutrient balance method: CK (recommended NPK fertilization), BC (NPK + biochar), OF (NPK + organic fertilizer), and BF (NPK + 1/2 biochar + 1/2 organic fertilizer). Using different fertilization strategies, the eco-stoichiometry characteristics of the soil microbial biomass and enzyme activity; the bioavailability of C, N, and P; and the differences in PY were investigated, and the direct and indirect effects of these factors on PY were determined over a two-year period. The results showed that exogenous organic matter input could considerably affect the stoichiometric ratios of soil microbial biomass; C; N; P; the stoichiometric ratios of C-converting, N-converting, and P-converting enzyme activities (expressed as BG+CBH, NAG+LAP, and AP, respectively); and the integrated enzyme index (IEI). The IEI was the highest in BF, followed by OF, BC, and CK. A significant positive correlation was found between the microbial biomass C, N, and P and their corresponding converting enzyme activities (p < 0.05). The ln(BG+CBH):ln(NAG+LAP), ln(BG+CBH):lnAP, and ln(NAG+LAP):lnAP ratios were all higher than 1:1, but they approached 1:1 in the order of CK-BC-OF-BF. Compared to soil C and N, P-converting enzyme activity was the primary limiting factor for soil nutrient conversion in the study area. BF was less restricted by P and more balanced in its nutrient ratio. The microbial biomass C:N:P could affect PY in eight ways. (1) Microbial biomass C:N directly decreased PY, and microbial biomass C:P indirectly increased PY. (2) It could decrease C-converting enzyme activity, (3) decrease N availability to increase C-converting enzyme activity, (4) decrease P availability, or (5) decrease P availability to decrease the soil C:P-converting enzyme activity ratio. Microbial biomass N:P indirectly increased PY (6) by increasing the soil C:P-converting enzyme activity ratio, (7) by increasing C-converting enzyme activity, or (8) by increasing N availability to increase C-converting enzyme activity. Thus, BF is an effective strategy for regulating the soil microbiological fertility index; enhancing C, N, and P nutrient conversion; and increasing PY. The input of exogenous organic matter can alter the stoichiometric ratios of soil microbial biomass C, N, and P; the stoichiometric ratios of C-converting, N-converting, and P-converting enzyme activities; and nutrient availability, thus regulating PY. Microbial biomass N:P and soil C:P-converting enzyme activity ratios influence PY the most. Full article

Microorganisms play a pivotal role in transforming and making phosphorus (P) available in soil through various mechanisms. However, their specific contributions to alleviating P limitation and enhancing P utilization efficiency in plants within the context of a P-deficient karst ecosystem remains unclear. In this study, eco-stoichiometric methods were employed to evaluate the P utilization efficiency of plants grown in the surveyed karst forest located in Guizhou Province, China. Metagenomic sequencing was utilized to further explore the functional genes and microorganisms involved in soil P cycling. The N:P ratio for 18 out of the 20 surveyed plants exceeded 16, indicating widespread P limitation in karst plants. Among them, plants with high P utilization efficiencies (Nandina domestica Thunb.; Mahonia bodinieri Gagnep.; Pyracantha fortuneana (Maxim.) Li) exhibited higher relative abundances of genes involved in soil P cycling compared to plants with low P utilization efficiencies (Tirpitzia sinensis (Hemsl.) Hallier f.; Albizia kalkora (Roxb.) Prain; Morella rubra Lour.), indicating greater potentials within their rhizosphere microbiomes for soil P transformation. The relative abundance of these functional genes had a significant and positive effect on plant P utilization efficiencies. Structural equation modeling further indicated that microbial P cycling gene abundance directly drove the increase in plant P utilization efficiencies. Specifically, genes involved in soil organic P mineralization (G6PD, suhB, phoD, ppx) and the P uptake and transform system (pstS, pstA, pstB, pstC) contributed to the enhancement of plant P utilization efficiencies. Soil microbial communities involved in P cycling were predominately attributed to Proteobacteria (45.16%–60.02%), Actinobacteria (9.45%–25.23%), and Acidobacteria (5.90%–9.85%), although their contributions varied among different plants. The rhizosphere functional microbial community can thus alleviate P limitation in karst plants, thereby enhancing plant P utilization efficiencies. This study investigated the strong synergism between karst plants and rhizosphere microorganisms and their associated underlying mechanisms from genetic and microbial perspectives. Full article

Ecological stoichiometry (ES) is an important index that reflects the balance of various elements in ecological processes. Therefore, it is of great significance to understand the soil nutrient cycle to clarify the environmental control of soil carbon (C), nitrogen (N), phosphorus (P), and potassium (K). In this study, we analyzed the spatial distribution of soil C, N, P, and K contents and the C:N:P:K stoichiometric characteristics of 0–20 cm and 20–40 cm of farmland and grassland in four agro-pastoral areas in Inner Mongolia. Spearman correlation was used to analyze the effects of environmental factors on the soil C:N:P:K stoichiometric relationship. The results showed that there was no fixed Redfield ratio for the soil stoichiometric relationship of farmland and grassland in Inner Mongolia, and the values were 15:2:1:9 to 145:10:1:26 and 25:1:1:29 to 228:15:1:65, respectively. The stoichiometric relationships between farmland and grassland were consistent with the law of geographical and spatial heterogeneity. The ratios of C:N, C:P, C:K, N:P, and N:K showed an N distribution from west to east, while the ratio of P:K showed a V distribution. The stoichiometric relationships in grassland soil were mainly affected by soil organic carbon and total nitrogen content, while those in farmland were mainly affected by total nitrogen and total phosphorus content. The annual mean precipitation has a significant effect on stoichiometric relationships in farmland, while the annual mean temperature has a more significant effect on grassland. In conclusion, the spatial distribution difference in the soil stoichiometric relationship in the agro-pastoral ecotone of Inner Mongolia was more significant than the difference in the land use pattern. The influences of annual mean temperature and annual mean precipitation on soil ecological stoichiometry were in accordance with the geographical spatial similarity law. Compared with grassland, the stoichiometric relationship of farmland soil was greatly affected by fertilization, and farmland in this region was mainly limited by carbon and nitrogen. Thus, field management should be carried out according to local conditions. This study is of great significance as it promotes the rational utilization of land resources and the sustainable development of agriculture. Full article

In order to explore the response of soil nutrient supply capacity and tea plant nutrient utilization capacity to tea-planting years in precious ancient tea garden, field investigation and indoor analysis methods were employed to research the soil and plant nutrient content, eco-stoichiometric characteristics and the correlation between them, with artificially bred Camellia tetracocca at different time periods (5 years, 15 years, 25 years and 40 years). The results showed that: (1) the contents of soil organic carbon and total nitrogen were higher in the 25- and 40-year teas than in 5- and 15-year teas. The soil pH and total phosphorus were the highest in the 40-year tea, and the available nutrient content was the lowest in the 40-year tea. (2) The contents of nitrogen, phosphorus and potassium in tea shoots were the highest in 15-year tea. The nutrient content of tea trees were highest according to the following order: new shoots > leaves > branches. (3) The N: P of soil and leaves was 4.11–7.55 and 6.37–11.76, respectively. Available nutrients and soil pH were the main factors affecting the contents of nitrogen, phosphorus and potassium in new shoots. In conclusion, the soil nutrient supply capacity and the nutrient utilization capacity of tea plants in the Camellia tetracocca garden were significantly different under different tea-planting years. The growth of the tea plants was restricted by the soil nitrogen supply. The nutrient absorption and utilization capacity of precious Camellia tetracocca were higher in the 15- and 25-year teas, respectively. The study provides the basis for the rational development and sustainable utilization of precious ancient tea plants, and the scientific management of tea gardens. Full article

The effects of environmental factors on topsoil nutrient distribution have been extensively discussed, but it remains unclear how they affect spatial characteristics of soil carbon (C), nitrogen (N), and phosphorus (P) stoichiometry at different depths. We collected 184 soil samples in the typical black soil region of northeast China. Ordinary kriging was performed to describe the spatial distribution of soil C, N, and P eco-stoichiometry. Redundancy analysis was used to explore relationships between C:N:P ratios and physicochemical characteristics. The soil classification was studied by hierarchical cluster analysis. The mean C, N, and P contents ranged from 15.67 to 20.08 g·kg⁻¹, 1.15 to 1.51 g·kg⁻¹, and 0.80 to 0.90 g·kg⁻¹ within measured depths. C, N, and P concentrations and stoichiometry increased from southwest to northeast, and the Songhua River was identified as an important transition zone. At 0–20 cm, soil water content explained most of the C, N, and P content levels and ratios in cluster 1, while latitude had the highest explanatory ability in cluster 2. For 20–40 cm, soil bulk density was the main influencing factor in both clusters. Our findings contribute to an improved knowledge of the balance and ecological interactions of C, N, and P in northeast China for its sustainability. Full article

Carbon, nitrogen, and phosphorus—nutrient and restrictive elements for plant growth and important components of the plant body—are mainly transferred and exchanged between plants and the soil environment. Changes in the carbon, nitrogen, and phosphorus eco-stoichiometry greatly impact the growth and expansion of Spartina alterniflora, and understanding these changes can reveal the nutrient coordination mechanism among ecosystem components. To explore the relationship between leaf and soil eco-stoichiometry and determine the key soil factors that affect leaf eco-stoichiometry, we collected leaf and soil samples of S. alterniflora at different tidal levels (i.e., 1, 3, and 5 km away from the coastline) in a coastal wetland in the Yancheng Elk Nature Reserve, Jiangsu province. We measured the leaf and soil carbon, nitrogen, and phosphorus contents and ratios, as well as the soil salinity and soil organic carbon. The results revealed the following. (1) The leaf stoichiometric characteristics and soil properties of S. alterniflora differed significantly between tidal levels; for example, total carbon, nitrogen, soil organic carbon were detected at their highest levels at 3 km and lowest levels at 5 km. (2) Significant correlations were detected between the leaf stoichiometric characteristics and soil characteristics. Additionally, nitrogen limitation was evident in the study area, as indicated by the nitrogen–phosphorus ratio being less than 14 and the soil nitrogen–phosphorus ratio being less than 1. (3) Soil salinity and the soil carbon–nitrogen ratio were shown to be the key factors that affect the eco-stoichiometric characteristics of S. alterniflora. These findings furthered our understanding of the nutrient distribution mechanisms and invasion strategy of S. alterniflora and can thus be used to guide S. alterniflora control policies formulated by government management departments in China. Full article

The effect of nitrogen (N) deposition on N limitation, phosphorus (P) limitation and the related soil and microbial stoichiometries remains unclear. A simulated nitrogen deposition (SND) experiment (control, ambient, medium and high) and molecular techniques (high-throughput sequencing of 16S and ITS) were conducted to examine the variations in abiotic and biotic properties and to describe the responses of microbial (bacteria and fungi) adaptation strategies in a moso bamboo (Phyllostachys edulis J. Houzeau) forest following SND. Soil water content (SWC) was positively correlated with the microbial community composition. Observed increases in total N and nitrate N contents and decreased ammonia N suggested that SND influenced nitrification. Chao1 and F:B showed that bacteria were more sensitive to SND than fungi. PCoA and linear discriminant analysis (LDA), coupled with effect size measurements (LefSe), confirmed that microbial community composition, including the subgroups (below class level), responded to SND by employing different adaptation strategies. Soil C:N indicated that the soil of the moso bamboo forest was under N limitation prior to SND. The increase in total P (TP), available P (AP) and microbial biomass P (MBP) suggested the acceleration of soil P cycling. Microbial biomass C (MBC) and microbial biomass N (MBN) were not affected by SND, which led to a significant shift in MBC:MBP and MBN:MBP, suggesting that P utilization per unit of C or N was promoted. There was a negative gradient correlation between the fungal community composition and MBC:MBP, while bacteria were positively correlated with MBN:MBP. The results illustrated that the response of fungi to MBC was more sensitive than that of bacteria in the process of accelerated P cycling, while bacteria were sensitive to MBN. Prior to P limitation, SND eliminated the soil N limitation and stimulated soil microorganisms to absorb more P, resulting in an increase in MBP, but did not alter MBC or MBN. This study contributes to our understanding of the adaptation strategies of fungi and bacteria and their responses to soil and microbial stoichiometries. Full article