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Keywords = collagen vitrigel

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16 pages, 733 KiB  
Review
Cryopreservation of Cell Sheets for Regenerative Therapy: Application of Vitrified Hydrogel Membranes
by Yoshitaka Miyamoto
Gels 2023, 9(4), 321; https://doi.org/10.3390/gels9040321 - 10 Apr 2023
Cited by 6 | Viewed by 4331
Abstract
Organ transplantation is the first and most effective treatment for missing or damaged tissues or organs. However, there is a need to establish an alternative treatment method for organ transplantation due to the shortage of donors and viral infections. Rheinwald and Green et [...] Read more.
Organ transplantation is the first and most effective treatment for missing or damaged tissues or organs. However, there is a need to establish an alternative treatment method for organ transplantation due to the shortage of donors and viral infections. Rheinwald and Green et al. established epidermal cell culture technology and successfully transplanted human-cultured skin into severely diseased patients. Eventually, artificial cell sheets of cultured skin were created, targeting various tissues and organs, including epithelial sheets, chondrocyte sheets, and myoblast cell sheets. These sheets have been successfully used for clinical applications. Extracellular matrix hydrogels (collagen, elastin, fibronectin, and laminin), thermoresponsive polymers, and vitrified hydrogel membranes have been used as scaffold materials to prepare cell sheets. Collagen is a major structural component of basement membranes and tissue scaffold proteins. Collagen hydrogel membranes (collagen vitrigel), created from collagen hydrogels through a vitrification process, are composed of high-density collagen fibers and are expected to be used as carriers for transplantation. In this review, the essential technologies for cell sheet implantation are described, including cell sheets, vitrified hydrogel membranes, and their cryopreservation applications in regenerative medicine. Full article
(This article belongs to the Special Issue Bioceramics, Bioglasses and Gels for Tissue Engineering)
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15 pages, 7940 KiB  
Article
Customizable Collagen Vitrigel Membranes and Preliminary Results in Corneal Engineering
by María Dolores Montalvo-Parra, Wendy Ortega-Lara, Denise Loya-García, Andrés Bustamante-Arias, Guillermo-Isaac Guerrero-Ramírez, Cesar E. Calzada-Rodríguez, Guiomar Farid Torres-Guerrero, Betsabé Hernández-Sedas, Italia Tatnaí Cárdenas-Rodríguez, Sergio E. Guevara-Quintanilla, Marcelo Salán-Gomez, Miguel Ángel Hernández-Delgado, Salvador Garza-González, Mayra G. Gamboa-Quintanilla, Luis Guillermo Villagómez-Valdez, Judith Zavala and Jorge E. Valdez-García
Polymers 2022, 14(17), 3556; https://doi.org/10.3390/polym14173556 - 29 Aug 2022
Cited by 5 | Viewed by 3459
Abstract
Corneal opacities are a leading cause of visual impairment that affect 4.2 million people annually. The current treatment is corneal transplantation, which is limited by tissue donor shortages. Corneal engineering aims to develop membranes that function as scaffolds in corneal cell transplantation. Here, [...] Read more.
Corneal opacities are a leading cause of visual impairment that affect 4.2 million people annually. The current treatment is corneal transplantation, which is limited by tissue donor shortages. Corneal engineering aims to develop membranes that function as scaffolds in corneal cell transplantation. Here, we describe a method for producing transplantable corneal constructs based on a collagen vitrigel (CVM) membrane and corneal endothelial cells (CECs). The CVMs were produced using increasing volumes of collagen type I: 1X (2.8 μL/mm2), 2X, and 3X. The vitrification process was performed at 40% relative humidity (RH) and 40 °C using a matryoshka-like system consisting of a shaking-oven harboring a desiccator with a saturated K2CO3 solution. The CVMs were characterized via SEM microscopy, cell adherence, FTIR, and manipulation in an ex vivo model. A pilot transplantation of the CECs/CVM construct in rabbits was also carried out. The thickness of the CVMs was 3.65–7.2 µm. The transparency was superior to a human cornea (92.6% = 1X; 94% = 2X; 89.21% = 3X). SEM microscopy showed a homogenous surface and laminar organization. The cell concentration seeded over the CVM increased threefold with no significant difference between 1X, 2X, and 3X (p = 0.323). The 2X-CVM was suitable for surgical manipulation in the ex vivo model. Constructs using the CECs/2X-CVM promoted corneal transparency restoration. Full article
(This article belongs to the Special Issue Multi-Functional Collagen-Based Biomaterials for Biomedical Applications)
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13 pages, 3303 KiB  
Article
Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption
by Emi Sano, Chihiro Mori, Naoki Matsuoka, Yuka Ozaki, Keisuke Yagi, Aya Wada, Koichi Tashima, Shinsuke Yamasaki, Kana Tanabe, Kayo Yano and Yu-suke Torisawa
Micromachines 2019, 10(11), 793; https://doi.org/10.3390/mi10110793 - 19 Nov 2019
Cited by 46 | Viewed by 6370
Abstract
Organs-on-chips are microfluidic devices typically fabricated from polydimethylsiloxane (PDMS). Since PDMS has many attractive properties including high optical clarity and compliance, PDMS is very useful for cell culture applications; however, PDMS possesses a significant drawback in that small hydrophobic molecules are strongly absorbed. [...] Read more.
Organs-on-chips are microfluidic devices typically fabricated from polydimethylsiloxane (PDMS). Since PDMS has many attractive properties including high optical clarity and compliance, PDMS is very useful for cell culture applications; however, PDMS possesses a significant drawback in that small hydrophobic molecules are strongly absorbed. This drawback hinders widespread use of PDMS-based devices for drug discovery and development. Here, we describe a microfluidic cell culture system made of a tetrafluoroethylene-propylene (FEPM) elastomer. We demonstrated that FEPM does not absorb small hydrophobic compounds including rhodamine B and three types of drugs, nifedipine, coumarin, and Bay K8644, whereas PDMS absorbs them strongly. The device consists of two FEPM layers of microchannels separated by a thin collagen vitrigel membrane. Since FEPM is flexible and biocompatible, this microfluidic device can be used to culture cells while applying mechanical strain. When human umbilical vein endothelial cells (HUVECs) were subjected to cyclic strain (~10%) for 4 h in this device, HUVECs reoriented and aligned perpendicularly in response to the cyclic stretch. Moreover, we demonstrated that this device can be used to replicate the epithelial–endothelial interface as well as to provide physiological mechanical strain and fluid flow. This method offers a robust platform to produce organs-on-chips for drug discovery and development. Full article
(This article belongs to the Special Issue Organs-on-chips)
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