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Keywords = colanic acid hydrolase

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17 pages, 4556 KiB  
Article
Combinatorial Metabolic Engineering and Enzymatic Catalysis Enable Efficient Production of Colanic Acid
by Suwei Li, Xianhao Xu, Xueqin Lv, Yanfeng Liu, Jianghua Li, Guocheng Du and Long Liu
Microorganisms 2022, 10(5), 877; https://doi.org/10.3390/microorganisms10050877 - 22 Apr 2022
Cited by 12 | Viewed by 3930
Abstract
Colanic acid can promote the lifespan of humans by regulating mitochondrial homeostasis, and it has widespread applications in the field of health. However, colanic acid is produced at a low temperature (20 °C) with low titer. Using Escherichia coli K-12 MG1655, we constructed [...] Read more.
Colanic acid can promote the lifespan of humans by regulating mitochondrial homeostasis, and it has widespread applications in the field of health. However, colanic acid is produced at a low temperature (20 °C) with low titer. Using Escherichia coli K-12 MG1655, we constructed the SRP-4 strain with high colanic acid production at 30 °C by enhancing the precursor supply and relieving the regulation of transcription for colanic acid synthesis genes by the RCS system. After media optimization, the colanic acid titer increased by 579.9-fold and reached 12.2 g/L. Subsequently, we successfully purified the colanic acid hydrolase and reduced the molecular weight of colanic acid (106.854 kDa), thereby eliminating the inhibition of high-molecular-weight colanic acid on strain growth. Finally, after adding the colanic acid hydrolase (4000 U/L), the colanic acid with low molecular weight reached 24.99 g/L in 3-L bioreactor, the highest titer reported so far. This high-producing strain of colanic acid will promote the application of low-molecular-weight colanic acid in the field of health. Full article
(This article belongs to the Special Issue Systems Metabolic Engineering of Industrial Microorganisms)
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