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Keywords = cedar (Juniperus phoenicea) essential oil

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17 pages, 3174 KiB  
Article
Preparation, Characterization, and Acetylcholinesterase Inhibitory Ability of the Inclusion Complex of β-Cyclodextrin–Cedar (Juniperus phoenicea) Essential Oil
by Eleni Kavetsou, Ioanna Pitterou, Annita Katopodi, Georgia Petridou, Abdelaziz Adjali, Spyros Grigorakis and Anastasia Detsi
Micro 2021, 1(2), 250-266; https://doi.org/10.3390/micro1020019 - 8 Dec 2021
Cited by 10 | Viewed by 3281
Abstract
The aim of the present study was the encapsulation of cedar (Juniperus phoenicea) essential oil (CEO) of Greek origin in β-cyclodextrin (β-CD) through the formation of inclusion complexes (ICs) using the co-precipitation method with different β-CD-to-CEO weight ratios (90:10, 85:15, 80:20, [...] Read more.
The aim of the present study was the encapsulation of cedar (Juniperus phoenicea) essential oil (CEO) of Greek origin in β-cyclodextrin (β-CD) through the formation of inclusion complexes (ICs) using the co-precipitation method with different β-CD-to-CEO weight ratios (90:10, 85:15, 80:20, 70:30 (w/w)). The encapsulation of CEO in β-CD through host–guest interactions was confirmed by Nuclear Magnetic Resonance (NMR) spectroscopy, FT-IR spectroscopy, Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA). The obtained ICs exhibited nanoscale size (315.9 nm to 769.6 nm),Polydispersity Index from 0.326 to 0.604 and satisfactory stability in suspension (−37.0 mV to −17.0 mV). The process yield was satisfactory, ranging between 65% and 78%, while the inclusion efficiency ranged from 10% to 27%. The in vitro release study conducted for the IC with the optimal characteristics (β-CD:CEO 80:20 (w/w)) exhibited a sustained release profile, with an initial burst effect in the first 5 h. The release profile could be well expressed by the Higuchi equation: Q = 18.893 t1/2 + 9.5919, R2 = 0.8491. The cedar EO presented significant acetylcholinesterase inhibition (IC50 37 μg/mL), which was prolonged by its encapsulation into the β-CD cavity. Full article
(This article belongs to the Section Microscale Materials Science)
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