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Keywords = benthic Euglena

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8 pages, 1214 KiB  
Article
Effect of pH on Photosynthesis of Euglena mutabilis Schmitz, an Acidophilic Benthic Flagellate
by Akira Haraguchi and Jiaojiao Zheng
Hydrobiology 2022, 1(1), 2-9; https://doi.org/10.3390/hydrobiology1010002 - 5 Oct 2021
Cited by 3 | Viewed by 5127
Abstract
The dependence of the photosynthetic rate of Euglena mutabilis Schmitz on pH across a range of 2.0–10.0 was investigated. Populations of E. mutabilis isolated from sediments of acidic mine drainage in Sensui (pH = 3.95) and a volcanic cold spring in Bougatsuru (pH [...] Read more.
The dependence of the photosynthetic rate of Euglena mutabilis Schmitz on pH across a range of 2.0–10.0 was investigated. Populations of E. mutabilis isolated from sediments of acidic mine drainage in Sensui (pH = 3.95) and a volcanic cold spring in Bougatsuru (pH = 5.32) were cultured in a pH-adjusted growth media for 96 h, and photosynthetic rate and dark respiration rate were measured. The maximum gross photosynthetic rate of E. mutabilis cells from the Sensui drainage population did not vary significantly over the pH range of 2.0–7.0, and their dark respiration rate showed high values at pH = 7.0. The maximum gross photosynthetic rate of E. mutabilis cells from the Bougatsuru spring population did not vary significantly within the pH range of 2.0–6.0, and their dark respiration rate tended to show high values at pH = 7.0. E. mutabilis can colonize under circumneutral conditions up to and including pH = 6.0. Full article
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8 pages, 1675 KiB  
Article
Ubiquity of Euglena mutabilis Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan
by Katsunori Yanagawa, Akira Haraguchi, Kai Yoshitake, Katsuhiro Asamatsu, Masanari Harano, Kei Yamashita and Jun-ichiro Ishibashi
Water 2021, 13(11), 1570; https://doi.org/10.3390/w13111570 - 1 Jun 2021
Cited by 7 | Viewed by 4029
Abstract
Three strains of Euglena mutabilis were isolated from sediments in acidic inland water systems (pH = 3.4–4.7), in Southwestern Japan—acid mine drainage in Sensui (Fukuoka), cold sulfidic spring in Bougatsuru (Oita), and a temporal pool in the Ebinokogen volcanic area (Miyazaki). All strains [...] Read more.
Three strains of Euglena mutabilis were isolated from sediments in acidic inland water systems (pH = 3.4–4.7), in Southwestern Japan—acid mine drainage in Sensui (Fukuoka), cold sulfidic spring in Bougatsuru (Oita), and a temporal pool in the Ebinokogen volcanic area (Miyazaki). All strains grew well in acidic media at pH 3.07. Phylogenetic analysis among these three strains showed high similarities to plastid SSU and nuclear SSU rRNA gene sequences (99.86% and 99.76%, respectively). They were closely related to the cultured isolates from other highly acidic habitats (pH = 2.0–5.9). Concentration of sulfate, aluminum, calcium, and iron had 7–70 fold of differences among the three studied habitats. Our results imply that the rRNA genes of E. mutabilis have compensated for their low genetic diversity by adapting to a wide pH range, as well as various water chemistry of habitats. Full article
(This article belongs to the Special Issue Algae: Indices of Water and Ecological Quality)
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