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Keywords = anti-serum against diapause hormone

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14 pages, 7543 KiB  
Article
Production of Transgenic Silkworm Using Anti-Serum Against Diapause Hormone in Diapause Strains of Silkworm, Bombyx mori
by Keiro Uchino, Megumi Sumitani, Tetsuya Iizuka and Hideki Sezutsu
Int. J. Mol. Sci. 2025, 26(15), 7604; https://doi.org/10.3390/ijms26157604 - 6 Aug 2025
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Abstract
In general, the silkworm, Bombyx mori, has a diapause trait in its eggs. Therefore, transgenic silkworm can be produced by embryonic microinjection using eggs laid by a non-diapause strain in B. mori. In this study, we performed microinjection using eggs of diapause [...] Read more.
In general, the silkworm, Bombyx mori, has a diapause trait in its eggs. Therefore, transgenic silkworm can be produced by embryonic microinjection using eggs laid by a non-diapause strain in B. mori. In this study, we performed microinjection using eggs of diapause strains which have good characteristics for industrial use, such as a big cocoon, thin and smooth silk, and tolerance against disease due to the growing industrial use of transgenic silkworms. For the conversion of egg diapause traits from diapause to non-diapause types, we used anti-serum against the diapause hormone of B. mori (BmDH), which was injected into maternal pupae, producing non-diapause eggs at a high rate. Finally, we attempted microinjection using three diapause strains with different voltinism (i.e., number of generations of an organism in a year) and were able to successfully produce transgenic silkworms in all three of them, demonstrating that our method is applicable to a wide range of silkworm strains with a diapause trait. Full article
(This article belongs to the Collection Feature Papers in “Molecular Biology”)
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