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During the past few decades, colorectal cancer (CRC) incidence and mortality have significantly increased, and CRC has become the leading cause of cancer-related death worldwide. Thus, exploring novel effective therapies for CRC is imperative. In this study, we investigated the effect of oncolytic adenovirus CD55-Smad4 on CRC cell growth. Cell viability assay, animal experiments, flow cytometric analysis, cell migration, and invasion assays, and Western blotting were used to detect the proliferation, apoptosis, migration, and invasion of CRC cells. The oncolytic adenovirus CD55-Smad4 was successfully constructed and effectively suppressed CRC cell proliferation in vivo and in vitro. Notably, CD55-Smad4 activated the caspase signaling pathway, inducing the apoptosis of CRC cells. Additionally, the generated oncolytic adenovirus significantly suppressed migration and invasion of CRC cells by overexpressing Smad4 and inhibiting Wnt/β-catenin/epithelial-mesenchymal transition (EMT) signaling pathway. Moreover, CRC cells treated with CD55-Smad4 formed less and smaller spheroid colonies in serum-free culture than cells in control groups, suggesting that CD55-Smad4 suppressed the stemness of CRC cells by inhibiting the Wnt/β-catenin pathway. Together, the results of this study provide valuable information for the development of a novel strategy for cancer-targeting gene-virotherapy and provide a deeper understanding of the critical significance of Smad4 in gene therapy of CRC. Full article

Examining relevant Daoist scriptures and records, this article traces the lineage relationship of Zhang Boduan (d. 1082) to his predecessors. His immediate teacher supposedly was Liu Haichan, based on whose teachings he compiled his main work, the Wuzhen pian (Awakening to Perfection). First outlined by the Song scholar Lu Sicheng, the story was later expanded in various collections of immortals’ biographies. It is well known that the Southern School of internal alchemy (Golden Elixir) was constructed by Bai Yuchan and his disciples in the early 13th century. I show that this centers on the claim that Zhang Boduan, as Bai’s forerunner, received his teachings from Liu Haichan, a line that was then expanded to include the immortals Zhongli Quan and Lü Dongbin. I also suggest that the alchemical teaching of the Zhong-Lü tradition is particularly characterized by its emphasis on the dual cultivation of inner nature and life-destiny, focusing on the key concepts of clarity and stillness as well as nonaction, while centering on the reverted elixir of the golden fluid. The teaching matches the Daode jing (Book of the Dao and Its Virtue) instructions to “empty the mind, fill the belly, weaken the will, and strengthen the bones” (ch. 3). This emphasis may well be the reason the Zhong-Lü tradition superseded the Twofold Mystery school flourishing in the Tang and rose to the fore. Full article