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Lentic ecosystems are highly vulnerable to anthropogenic disturbances, which can lead to ecological imbalances with the loss of native biodiversity. This research evaluated the impact of anthropogenic activities on water quality and the trophic status of the Pacucha Lagoon, Andahuaylas, Apurimac. Seasonal sampling was conducted at six strategic points on the surface and bottom of the lake, following standardized protocols, during both the dry and rainy seasons. Physical and chemical parameters, metal ions, chlorophyll-a, and trophic status were determined using the Carlson indices, and water quality was assessed through the WQI-PE. The quantification of anthropogenic impacts was performed using the Leopold matrix. Statistical analysis included descriptive methods, ANOVA, Pearson correlation, and PCA at 5% significance. The results classify the lake as having “Regular” water quality according to WQI-PE. Physical and chemical parameters remain within the Environmental Quality Standards (ECA-Peru), while heavy metal ions exceed the limits in both seasons. The system exhibits mesotrophic conditions that tend to become eutrophic during the rainy season, suggesting a favorable nutrient balance for biological productivity. Activities with the most significant adverse impacts include agricultural practices that involve the intensive use of agrochemicals, morphological alterations to the surrounding area, and unregulated aquaculture. The lack of effective regulatory mechanisms and a lack of commitment to conservation suggest a high risk of irreversible degradation of the Pacucha Lagoon ecosystem. Full article