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Keywords = WI38VA13 subcloned 2RA cells

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9 pages, 681 KiB  
Article
Expression of Heat Shock Proteins in Human Fibroblast Cells under Magnetic Resonant Coupling Wireless Power Transfer
by Kohei Mizuno, Naoki Shinohara and Junji Miyakoshi
Energies 2015, 8(10), 12020-12028; https://doi.org/10.3390/en81012020 - 22 Oct 2015
Cited by 1 | Viewed by 5992
Abstract
Since 2007, resonant coupling wireless power transfer (WPT) technology has been attracting attention and has been widely researched for practical use. Moreover, dosimetric evaluation has also been discussed to evaluate the potential health risks of the electromagnetic field from this WPT technology based [...] Read more.
Since 2007, resonant coupling wireless power transfer (WPT) technology has been attracting attention and has been widely researched for practical use. Moreover, dosimetric evaluation has also been discussed to evaluate the potential health risks of the electromagnetic field from this WPT technology based on the International Commission on Non-Ionizing Radiation Protection (ICNIRP) guidelines. However, there has not been much experimental evaluation of the potential health risks of this WPT technology. In this study, to evaluate whether magnetic resonant coupling WPT induces cellular stress, we focused on heat shock proteins (Hsps) and determined the expression level of Hsps 27, 70 and 90 in WI38VA13 subcloned 2RA human fibroblast cells using a western blotting method. The expression level of Hsps under conditions of magnetic resonant coupling WPT for 24 h was not significantly different compared with control cells, although the expression level of Hsps for cells exposed to heat stress conditions was significantly increased. These results suggested that exposure to magnetic resonant coupling WPT did not cause detectable cell stress. Full article
(This article belongs to the Special Issue Wireless Power Transfer)
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11 pages, 1231 KiB  
Article
In Vitro Evaluation of Genotoxic Effects under Magnetic Resonant Coupling Wireless Power Transfer
by Kohei Mizuno, Naoki Shinohara and Junji Miyakoshi
Int. J. Environ. Res. Public Health 2015, 12(4), 3853-3863; https://doi.org/10.3390/ijerph120403853 - 7 Apr 2015
Cited by 9 | Viewed by 6473
Abstract
Wireless power transfer (WPT) technology using the resonant coupling phenomenon has been widely studied, but there are very few studies concerning the possible relationship between WPT exposure and human health. In this study, we investigated whether exposure to magnetic resonant coupling WPT has [...] Read more.
Wireless power transfer (WPT) technology using the resonant coupling phenomenon has been widely studied, but there are very few studies concerning the possible relationship between WPT exposure and human health. In this study, we investigated whether exposure to magnetic resonant coupling WPT has genotoxic effects on WI38VA13 subcloned 2RA human fibroblast cells. WPT exposure was performed using a helical coil-based exposure system designed to transfer power with 85.4% efficiency at a 12.5-MHz resonant frequency. The magnetic field at the positions of the cell culture dishes is approximately twice the reference level for occupational exposure as stated in the International Commission on Non-Ionizing Radiation Protection (ICNIRP) guidelines. The specific absorption rate at the positions of the cell culture dishes matches the respective reference levels stated in the ICNIRP guidelines. For assessment of genotoxicity, we studied cell growth, cell cycle distribution, DNA strand breaks using the comet assay, micronucleus formation, and hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene mutation, and did not detect any significant effects between the WPT-exposed cells and control cells. Our results suggest that WPT exposure under the conditions of the ICNIRP guidelines does not cause detectable cellular genotoxicity. Full article
(This article belongs to the Special Issue Electromagnetic Fields and Health)
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