Fungal secondary metabolites (SMs) have broad applications in biomedicine, biocontrol, and the food industry. In this study, whole-genome sequencing and annotation of
Taiwanofungus gaoligongensis were conducted, followed by comparative genomic analysis with 11 other species of Polyporales to examine genomic variations and secondary metabolite biosynthesis pathways. Additionally, transcriptome data were used to analyze the differential expression of polyketide synthase (PKS), terpene synthase (TPS) genes, and transcription factors (TFs) under different culture conditions. The results show that
T. gaoligongensis differs from other fungal species in genome size (34.58 Mb) and GC content (50.72%). The antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) analysis reveals significant variation in the number of SM biosynthetic gene clusters (SMBGCs) across the 12 species (12–29), with
T. gaoligongensis containing 25 SMBGCs: 4 PKS, 6 non-ribosomal peptide synthetase (NRPS), and 15 TPS clusters. The
TgPKS1 gene is hypothesized to be involved in the biosynthesis of orsellinic acid or its derivatives, while
TgPKS2 might catalyze the synthesis of 6-methylsalicylic acid (6MSA) and its derivatives. The
TgTRI5 genes are suggested to synthesize tetracyclic sesquiterpene type B trichothecene compounds, while
TgPentS may be involved in the synthesis of δ-cadinol, β-copaene, and α-murolene analogs or derivatives. Comparative genomic analysis shows that the genome size of
T. gaoligongensis is similar to that of
T. camphoratus, with comparable SMs. Both species share four types of PKS domains and five distinct types of TPS. Additionally,
T. gaoligongensis exhibits a high degree of similarity to
Laetiporus sulphureus, despite belonging to a different genus within the same family. Transcriptome analysis reveals significant variation in the expression levels of PKS and TPS genes across different cultivation conditions. The
TgPKS1 and
TgPKS4 genes, along with nine
TgTFs, are significantly upregulated under three solid culture conditions. In contrast, under three different liquid culture conditions, the
TgPKS3,
TgTRI5-1, and
TgTRI5-2 genes, along with twelve
TgTFs, exhibit higher activity. Co-expression network analysis and
TgTFs binding site prediction in the promoter regions of
TgPKS and
TgTPS genes suggest that
TgMYB9 and
TgFTD4 regulate
TgPKS4 expression.
TgHOX1,
TgHSF2,
TgHSF3, and
TgZnF4 likely modulate
TgPKS3 transcriptional activity.
TgTRI5-1 and
TgTRI5-5 expression is likely regulated by
TgbZIP2 and
TgZnF15, respectively. This study provides new insights into the regulatory mechanisms of SMs in
T. gaoligongensis and offers potential strategies for enhancing the biosynthesis of target compounds through artificial intervention.
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