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Keywords = Streptomyces jeddahensis

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19 pages, 2931 KiB  
Article
Lipid Production in Streptomyces jeddahensis Is Enhanced by Glucose and Fatty Acid Derivatives, with Temperature Variations Influencing Gene Expression and Biosynthesis
by Pamella Apriliana, Prihardi Kahar, Nova Rachmadona, Witta Kartika Restu, Akihiko Kondo and Chiaki Ogino
Fermentation 2025, 11(2), 45; https://doi.org/10.3390/fermentation11020045 - 21 Jan 2025
Cited by 1 | Viewed by 1551
Abstract
The potential of Streptomyces jeddahensis for lipid production was investigated, focusing on its ability to utilize various carbon sources under different temperature conditions. Glucose and fatty acid derivatives (sodium hexanoate, sodium octanoate, and sodium decanoate) were tested at 30 °C, 37 °C, and [...] Read more.
The potential of Streptomyces jeddahensis for lipid production was investigated, focusing on its ability to utilize various carbon sources under different temperature conditions. Glucose and fatty acid derivatives (sodium hexanoate, sodium octanoate, and sodium decanoate) were tested at 30 °C, 37 °C, and 45 °C to optimize growth and lipid biosynthesis. The results revealed that 37 °C was the optimal temperature for lipid accumulation, with sodium octanoate leading to the highest lipid content. While growth declined at 45 °C, significant lipid production persisted, indicating an adaptive stress response. Gene expression analysis identified upregulation of key metabolic genes, including STSP_03080 (DGAT) and STSP_67970 (fabD), under thermal stress, highlighting enhanced fatty acid biosynthesis pathways. This study demonstrates the capacity of S. jeddahensis to efficiently convert medium-chain fatty acids into lipids, supporting its use as a sustainable and scalable platform for biodiesel production and other lipid-based industrial applications. Full article
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12 pages, 593 KiB  
Article
Microbial Fermentation of the Water-Soluble Fraction of Brewers’ Spent Grain for the Production of High-Value Fatty Acids
by Stefano Serra, Paola D’Arrigo, Letizia Anna Maria Rossato and Eleonora Ruffini
Fermentation 2023, 9(12), 1008; https://doi.org/10.3390/fermentation9121008 - 7 Dec 2023
Cited by 4 | Viewed by 2445
Abstract
Brewers’ spent grain (BSG) constitutes the primary by-product of the brewing industry. The valorization of BSG from a circular economy perspective is of high industrial interest. The objective of this study was the exploitation of BSG for the microbial production of branched-chain fatty [...] Read more.
Brewers’ spent grain (BSG) constitutes the primary by-product of the brewing industry. The valorization of BSG from a circular economy perspective is of high industrial interest. The objective of this study was the exploitation of BSG for the microbial production of branched-chain fatty acids (BCFAs) and polyunsaturated fatty acids (PUFAs), representing two different classes of high-value fatty acids (FAs). In the present study, this waste material underwent treatment with hot water in an autoclave and the resultant extract was utilized for the preparation of a novel liquid medium (BSG medium) to be employed for microbial fermentation. Screening and subsequent scaling-up experiments confirmed the suitability of the BSG medium to support the microbial production of various high-value FAs. In particular, Streptomyces jeddahensis and Conidiobolus heterosporus could be employed for BCFAs production, Pythium ultimum and Mortierella alpina could be used to provide cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and arachidonic acid (ARA), whereas Mucor circinelloides, when grown in a BSG medium, was able to accumulate γ-linolenic acid (GLA). Full article
(This article belongs to the Special Issue Recent Advances in Bioconversion of Biomass to Value-Added Products)
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