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Search Results (2)

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Keywords = Rana catesbeianus

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13 pages, 1412 KB  
Article
Transport of American Bullfrogs with Moistened Foam and without Foam: Plasma Biochemistry and Erythrogram Responses
by Adriana Xavier Alves, Nayara Netto dos Santos, Gean Paulo Andrade Reis, Mariele Lana, Bruno Dias dos Santos, Ragli Oliveira Azevedo, Renan Rosa Paulino, Frederico Augusto de Alcântara Costa, Daniel Abreu Vasconcelos Campelo and Galileu Crovatto Veras
Fishes 2024, 9(9), 347; https://doi.org/10.3390/fishes9090347 - 31 Aug 2024
Viewed by 1760
Abstract
This study aimed to evaluate two transportation methods (with moistened foam and without foam) for 10 h on blood parameters of bullfrogs 0, 6, 12, 24, and 48 h after transportation. There was no mortality. The glucose increased at 0 and 12 h [...] Read more.
This study aimed to evaluate two transportation methods (with moistened foam and without foam) for 10 h on blood parameters of bullfrogs 0, 6, 12, 24, and 48 h after transportation. There was no mortality. The glucose increased at 0 and 12 h after transportation and returned to baseline at 24 h in both transportations. Triglycerides increased at 0 and 6 h in both transportations and were restored 12 h after transport with foam and 24 h in transport without foam. Plasma proteins and globulins increased at 0 h after transportation under both transportations. After 48 h, there was a reduction in transport without foam. Globulins decreased 48 h under both transportations. Albumin increased at 12, 24, and 48 h after both transportations. Transport with foam had high albumin. The albumin/globulin ratio increased 24 and 48 h after both transportations. The number of erythrocytes increased at 0 h and recovered after 6 h in transport with foam and 12 h in transport without foam. Hematocrit and hemoglobin increased at 0 h and recovered at 6 h in both transportations. MCV increased 48 h after transportation with foam. MCHC decreased 12, 24, and 48 h after both transportations. MCH was lower in the transport carried out with foam. Full article
(This article belongs to the Special Issue Physiological Response Mechanisms of Aquatic Animals to Stress)
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12 pages, 1561 KB  
Article
Indirect Competitive Enzyme-Linked Immunosorbent Assay Based on Broad-Spectrum Antibody for Simultaneous Determination of Thirteen Fluoroquinolone Antibiotics in Rana catesbeianus
by Biao Zhang, Yihan Lang, Bowen Guo, Zhengyang Cao, Jin Cheng, Danfeng Cai, Xuping Shentu and Xiaoping Yu
Foods 2023, 12(13), 2530; https://doi.org/10.3390/foods12132530 - 29 Jun 2023
Cited by 39 | Viewed by 2494
Abstract
Fluoroquinolone (FQ) is a type of widely used antibiotic in agriculture and aquaculture, and exposure to low doses of FQs may result in the transfer of resistance between animal and human pathogens. Based on the optimization of the operating parameters, an indirect competitive [...] Read more.
Fluoroquinolone (FQ) is a type of widely used antibiotic in agriculture and aquaculture, and exposure to low doses of FQs may result in the transfer of resistance between animal and human pathogens. Based on the optimization of the operating parameters, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) standard curve was constructed for the simultaneous detection of 13 FQs, including enrofloxacin (ENR), ciprofloxacin (CIP), sarafloxacin (SAR), ofloxacin (OFL), norfloxacin (NOR), pefloxacin mesylate (PM), pefloxacin (PEF), enoxacin (ENX), marbofloxacin (MAR), fleroxacin (FLE), lomefloxacin (LOM), danofloxacin (DAN), and difloxacin (DIF). The limit of detection (LOD, computed as IC10) and sensitivity (IC50) of the ic-ELISA for ENR were 0.59 μg/L and 19.23 μg/L, respectively. The precision and dependability of the detection results of this ic-ELISA were properly verified by HPLC in Rana catesbeianus samples. This indicated that the established ic-ELISA approach could be utilized to determine the FQs in Rana catesbeianus. In addition, this ic-ELISA, based on a broad-spectrum antibody, provides a technical reference and potential strategy for an immunoassay of hazard factors with similar structure. Full article
(This article belongs to the Special Issue The Generation and Control of Harmful Products in Food Processing)
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