Search Results (3)

Background/Objectives: Metallic NPs have been explored for various therapeutic uses owing to utilitarian physicochemical characteristics such as antibacterial, anti-inflammatory, and healing properties. The objective of this study is to evaluate the therapeutic potential of novel silver carbonate nanostructures in promoting wound healing and their antibacterial activity against Pseudomonas chengduensis and Staphylococcus aureus. Methods: In this work, we prepared Ag₂CO₃ nanoparticles through a two-step methodology that was expected to improve the material’s biomedical performance and biocompatibility. The characterization and assessment of synthesized NPs biocompatibility were conducted using hemolysis assays on the blood of a healthy male human. Further, we performed molecular docking analysis to confirm interactions of silver NPs with biological molecules. Results: In detail, the synthesized NPs showed <5% hemolysis activity at various concentrations, confirming their therapeutic applicability. Additionally, the NPs had good metabolic activities; they raised the T3/T4 hormone content and acted effectively on Insulin-like Growth Factor 1 (IGF-1) in diabetic models. They also facilitated the rate of repair by having the diabetic wounds reach 100% re-epithelialization by day 13, unlike the control group, which reached the same level only by day 16. The synthesized Ag₂CO₃ NPs exhibited high antimicrobial potential against both Pseudomonas chengduensis and Staphylococcus aureus, hence being a potential material that can be used for infection control in biomedical tissue engineering applications. Conclusions: These findings concluded that novel synthesis methods lead to the formation of NPs with higher therapeutic prospects; however, studies of their metaphysical and endocrinological effects are necessary. Full article

Pseudomonas chengduensis is a new species of Pseudomonas discovered in 2014, and currently, there is a scarcity of research on this bacterium. The P. chengduensis strain WD211 was isolated from a fish pond. This study investigated the purification capability and environmental adaptability of strain WD211 in wastewater and described the basic features and functional genes of its complete genome. According to the results, the sewage treated with strain WD211 showed a decrease in concentration of 18.12% in total nitrogen, 89.39% in NH₄⁺, 62.16% in NO₃⁻, 79.97% in total phosphorus, and 71.41% in COD after 24 h. Strain WD211 is able to survive in a pH range of 6–11. It shows resistance to 7% sodium chloride and different types of antibiotics. Genomic analysis showed that strain WD211 may remove nitrogen and phosphorus through the metabolic pathway of nitrogen assimilation and phosphorus accumulation, and that it can promote organic decomposition through oxygenase. Strain WD211 possesses genes for producing betaine, trehalose, and sodium ion transport, which provide it with salt tolerance. It also has genes for antibiotic efflux and multiple oxidases, which give it antibiotic resistance. This study contributes to the understanding of the sewage treatment ability and potential applications of P. chengduensis. Full article

Four strains of bioflocculant-producing bacteria were isolated from a palm oil mill effluent (POME). The four bacterial strains were identified as Pseudomonas alcaliphila (B1), Pseudomonas oleovorans (B2), Pseudomonas chengduensis (B3), and Bacillus nitratireducens (B4) by molecular identification. Among the four bacterial strains, Bacillus nitratireducens (B4) achieved the highest flocculating activity (49.15%) towards kaolin clay suspension after eight hours of cultivation time and was selected for further studies. The optimum conditions for Eriochrome Black T (EBT) flocculation regarding initial pH, type of cation, and B4 dosage were determined to be pH 2, Ca²⁺ cations, and a dosage of 250 mL/L of nutrient broth containing B4. Under these conditions, above 90% of EBT dye removal was attained. Fourier transform infrared spectroscopic (FT-IR) analysis of the bioflocculant revealed the presence of hydroxyl, alkyl, carboxyl, and amino groups. This bioflocculant was demonstrated to possess a good flocculating activity, being a promissory, low-cost, harmless, and environmentally friendly alternative for the treatment of effluents contaminated with dyes. Full article