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Keywords = Phaeosphaeriopsis sp.

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11 pages, 1400 KiB  
Article
New Isocoumarins from the Marine Fungus Phaeosphaeriopsis sp. WP-26
by Pei Wang, Huifang Wang, Juchun Yang, Li Yang, Caihong Cai, Jingzhe Yuan, Fei Wu, Cuijuan Gai, Wenli Mei and Haofu Dai
Mar. Drugs 2023, 21(3), 150; https://doi.org/10.3390/md21030150 - 25 Feb 2023
Cited by 5 | Viewed by 2590
Abstract
Five new isocoumarins, phaeosphaerins A–E (15), were isolated from the fermentation broth of the marine fungus Phaeosphaeriopsis sp. WP-26, along with one known isocoumarin, 6,8-dihydroxy-7-methoxy-3-methylisocoumarin (6), and two known pimarane-type diterpenes, diaportheins A (7) and [...] Read more.
Five new isocoumarins, phaeosphaerins A–E (15), were isolated from the fermentation broth of the marine fungus Phaeosphaeriopsis sp. WP-26, along with one known isocoumarin, 6,8-dihydroxy-7-methoxy-3-methylisocoumarin (6), and two known pimarane-type diterpenes, diaportheins A (7) and B (8). Their structures were elucidated via NMR experiments, X-ray diffraction analysis, and comparison of the experimental and computed ECD curves. Compounds 17 displayed weak neuroprotective effects against H2O2-induced damage in SH-SY5Y cells. Moreover, compound 8 showed cytotoxicity against BEL-7402, SGC-7901, K562, A549, and HL-60 cell lines. Full article
(This article belongs to the Section Structural Studies on Marine Natural Products)
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11 pages, 1316 KiB  
Article
Phaeosphamides A and B, Cytotoxic Cyclodecadepsipeptides from the Mangrove-Derived Fungus Phaeosphaeriopsis sp. S296
by Siwen Niu, Jianlin He, Shuhuan Huang, Shouyuan Wu, Ling Zeng, Juan Wang, Bihong Hong and Ziming Chen
Mar. Drugs 2022, 20(10), 591; https://doi.org/10.3390/md20100591 - 21 Sep 2022
Cited by 6 | Viewed by 2496
Abstract
Chemical examination of the fermented broth of the mangrove-derived fungus Phaeosphaeriopsis sp. S296 resulted in the isolation of two new cyclodecadepsipeptides, namely phaeosphamides A (1) and B (2), as well as one known congener Sch 217048 (3). [...] Read more.
Chemical examination of the fermented broth of the mangrove-derived fungus Phaeosphaeriopsis sp. S296 resulted in the isolation of two new cyclodecadepsipeptides, namely phaeosphamides A (1) and B (2), as well as one known congener Sch 217048 (3). The structures of new metabolites, including absolute configurations, were established on the basis of extensive spectroscopic data analyses, chemical conversion, and Marfey’s method. The 2-hydroxy-3-methylpentanoic acid (Hmp) moiety and pipecolic acid (Pip) unit in structures were rarely discovered in nature. Interestingly, compounds 13 are examples of peptides discovered from the fungal genus Phaeosphaeriopsis for the first time. All identified compounds were evaluated for their cytotoxicity against five tumor cell lines of AGS, BEL-7402, HepG2, B16, and BIU87. Among them, compound 1 showed inhibitory activities against these tumor cell lines with IC50 values ranging from 5.14 to 66.38 μM. A further mechanistic investigation found that 1 arrested AGS cells in the G2 phase and induced their apoptosis in a dose-dependent manner. Full article
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