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Keywords = Pantoea rodasii

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8 pages, 288 KiB  
Article
Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone
by Nina Yusrina Muhamad Yunos, Wen-Si Tan, Nur Izzati Mohamad, Pui-Wan Tan, Tan-Guan-Sheng Adrian, Wai-Fong Yin and Kok-Gan Chan
Sensors 2014, 14(5), 9145-9152; https://doi.org/10.3390/s140509145 - 23 May 2014
Cited by 6 | Viewed by 6854
Abstract
Proteobacteria use quorum sensing to regulate target gene expression in response to population density. Quorum sensing (QS) is achieved via so-called signalling molecules and the best-studied QS signalling system uses N-acyl homoserine lactones (AHLs). This study aimed to identify and characterize the [...] Read more.
Proteobacteria use quorum sensing to regulate target gene expression in response to population density. Quorum sensing (QS) is achieved via so-called signalling molecules and the best-studied QS signalling system uses N-acyl homoserine lactones (AHLs). This study aimed to identify and characterize the production of AHLs by a bacterium ND03 isolated from a Malaysian tropical rainforest waterfall. Molecular identification showed that ND03 is a Pantoea sp. closely related to Pantoea rodasii. We used Chromobacterium violaceum CV026, an AHL biosensor for preliminary AHL production screening and then used high resolution triple quadrupole liquid chromatography-mass spectrometry, to confirm that P. rodasii strain ND03 produced N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL). To the best of our knowledge, this is the first report for such a discovery in P. rodasii strain ND03. Full article
(This article belongs to the Section Biosensors)
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