In this study, in order to protect the characteristic
Dendrobium devonianum Paxt industry in the Longling area, and promote the healthy development of its characteristic Chinese herbal medicines in Yunnan Province, China, the identification of
Dendrobium devonianum Paxt and
Dendrobium officinale Kimura et
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In this study, in order to protect the characteristic
Dendrobium devonianum Paxt industry in the Longling area, and promote the healthy development of its characteristic Chinese herbal medicines in Yunnan Province, China, the identification of
Dendrobium devonianum Paxt and
Dendrobium officinale Kimura et Migo from Longling county was discussed using time of flight mass spectrometry. The data of 13
Dendrobium devonianum and 7
Dendrobium officinale in the Longling area were collected by TOF MS-IDA-15 MS/MS mode, and the collected data were analyzed by PCA and T-test using MarkerView software, and the difference markers were searched using the database to confirm their compound structures. In positive and negative ion modes, 3645 and 2344 peaks were detected, respectively; 64 positive ion compounds and 60 negative ion compounds, for a total of 124 compounds were identified, mainly including organic acids, polyphenols, alkaloids, amino acids and their derivatives, benzene and its derivatives, and other compounds. The score plot and loading plot analyzed by PCA show that
Dendrobium devonianum and
Dendrobium officinale collected in the Longling area can be effectively identified and differentiated by high-resolution mass spectrometry with the 15 different markers in positive ion mode and 17 markers in negative ion mode, respectively. The successful identification of
Dendrobium devonianum and
Dendrobium officinale fully demonstrates that TOF MS can be effectively used in the identification of
Dendrobium and related Chinese herbal medicines with broadly application foreground.
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