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Keywords = Infectious Bovine Keratoconjunctivosous (IBK)

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9 pages, 1981 KiB  
Article
Sphingolipidomics of Bovine Pink Eye: A Pilot Study
by Paul L. Wood and Lynda M. J. Miller
Vet. Sci. 2022, 9(8), 388; https://doi.org/10.3390/vetsci9080388 - 28 Jul 2022
Cited by 1 | Viewed by 2244
Abstract
Sphingolipids are essential structural components of tear film that protect the surface of the eye from dehydration. A detailed analysis of the effects of pink eye infections on the sphingolipidome in cattle has not previously been undertaken. We recently published a new assay [...] Read more.
Sphingolipids are essential structural components of tear film that protect the surface of the eye from dehydration. A detailed analysis of the effects of pink eye infections on the sphingolipidome in cattle has not previously been undertaken. We recently published a new assay utilizing high-resolution mass spectrometric monitoring of the chloride adducts of sphingolipids that provides enhanced sensitivity and specificity. Utilizing this assay, we monitored decreases in the levels of tear film ceramides with short-chain fatty acids, hydroxy-ceramides, phytoceramides, and hydroxy-phytoceramides. Dihydroceramide levels were unaltered and increased levels of ceramides with long-chain fatty acids (24:0 and 24:1) were monitored in cattle with pink eye. The data from this pilot study (n = 8 controls and 8 pink eye) demonstrate a major disruption of the lipid tear film layer in pink eye disease, that can result in severe eye irritation and damage. Full article
(This article belongs to the Section Veterinary Biomedical Sciences)
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