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Keywords = IR laser inactivation

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10 pages, 2474 KB  
Article
FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation
by Irina Saraeva, Eteri Tolordava, Svetlana Sheligyna, Alyona Nastulyavichus, Roman Khmelnitskii, Nikolay Pokryshkin, Dmitriy Khmelenin, Sergey Kudryashov, Andrey Ionin and Andrey Akhmatkhanov
Int. J. Mol. Sci. 2023, 24(6), 5119; https://doi.org/10.3390/ijms24065119 - 7 Mar 2023
Cited by 12 | Viewed by 2626
Abstract
We report the successful inactivation of P. aeruginosa strain by femtosecond infrared (IR) laser radiation at the resonant wavelengths of 3.15 μm and 6.04 μm, chosen due to the presence of characteristic molecular vibrations in the main structural elements of the bacterial cells [...] Read more.
We report the successful inactivation of P. aeruginosa strain by femtosecond infrared (IR) laser radiation at the resonant wavelengths of 3.15 μm and 6.04 μm, chosen due to the presence of characteristic molecular vibrations in the main structural elements of the bacterial cells in these spectral ranges: vibrations of amide groups in proteins (1500–1700 cm−1), and C-H vibrations in membrane proteins and lipids (2800–3000 cm−1). The underlying bactericidal structural molecular changes were revealed by the stationary Fourier-transform IR spectroscopy, with the spectral peaks parameters being obtained by Lorentzian fitting with the hidden peaks revealed by the second derivative calculations, while no visible damage to the cell membranes was identified by scanning and transmission electron microscopy. Full article
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