Search Results (2)

Natural product-based structural templates have immensely shaped small molecule drug discovery, and new biogenic natural products have randomly provided the leads and molecular targets in anti-analgesic activity spheres. Pain relief achieved through opiates and non-steroidal anti-inflammatory drugs (NSAIDs) has been under constant scrutiny owing to their tolerance, dependency, and other organs toxicities and tissue damage, including harm to the gastrointestinal tract (GIT) and renal tissues. A new, 3′,4′,6′-triacetylated-glucoside, 2-O-β-D-(3′,4′,6′-tri-acetyl)-glucopyranosyl-3-methyl pentanoic acid was obtained from Ficus populifolia, and characterized through a detailed NMR spectroscopic analysis, i.e., ¹H-NMR, ¹³C-DEPT-135, and the 2D nuclear magnetic resonance (NMR) correlations. The product was in silico investigated for its analgesic prowess, COX-2 binding feasibility and scores, drug likeliness, ADMET (absorption, distribution, metabolism, excretion, and toxicity) properties, possible biosystem’s toxicity using the Discovery Studio^®, and other molecular studies computational software programs. The glycosidic product showed strong potential as an analgesic agent. However, an in vivo evaluation, though at strong levels of pain-relieving action, was estimated on the compound’s extract owing to the quantity and yield issues of the glycosidic product. Nonetheless, the F. populifolia extract showed the analgesic potency in eight-week-old male mice on day seven of the administration of the extract’s dose in acetic acid-induced writhing and hot-plate methods. Acetic acid-induced abdominal writhing for all the treated groups decreased significantly (p < 0.0001), as compared to the control group (n = 6) by 62.9%, 67.9%, and 70.9% of a dose of 100 mg/kg (n = 6), 200 mg/kg (n = 6), and 400 mg/kg (n = 6), respectively. Similarly, using the analgesia meter, the reaction time to pain sensation increased significantly (p < 0.0001), as compared to the control (n = 6). The findings indicated peripheral and central-nervous-system-mediated analgesic action of the product obtained from the corresponding extract. Full article

The Ficus populifolia Vahl. in the Arabian Peninsula is threatened, peripheral, and geographically isolated from its main population in Africa. Here, the entire plastome of F. populifolia from the Arabian Peninsula was sequenced and analyzed to provide a baseline genetic resource for future research. The F. populifolia plastome has a classic quadripartite structure with a size of 160,610 bp, the large and small single copies of 88,729 and 20,097 bp, respectively, and each pair of inverted repeats are 25,892 bp. The genome includes 113 unique genes, 79 protein-coding genes, 30 tRNAs, and 4 rRNAs. The results reveal a total of 49 long repeats, including (30) palindromic, (14) forward, and (5) reverse repeats. Similarly, a total of 186 simple sequence repeats were identified, 83.8% of which were mononucleotides. The genomic comparison with four Ficus species indicated that the plastome of F. populifolia was highly conserved, with some hypervariable noncoding regions. The phylogenomic analysis of 28 species of Ficus, based on 78 coding genes, revealed that F. populifolia is closely related to the African species F. lyrata. The genomic data generated in this study provide valuable resources for future investigations on the population genetics, authentication, and genetic conservation of the wild Arabian population of F. populifolia. Full article