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Keywords = Faustoviruses

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10 pages, 7364 KB  
Article
Diversity of Amoeba-Associated Giant Viruses Isolated in Algeria
by Hadjer Boudjemaa, Julien Andreani, Idir Bitam and Bernard La Scola
Diversity 2020, 12(6), 215; https://doi.org/10.3390/d12060215 - 29 May 2020
Cited by 6 | Viewed by 3969
Abstract
The discovery of several giant amoeba viruses has opened up a novel area in the field of virology. Despite this, knowledge about ecology of these viruses remains patchy. In this study, we aimed to characterize the diversity of giant viruses in Algeria by [...] Read more.
The discovery of several giant amoeba viruses has opened up a novel area in the field of virology. Despite this, knowledge about ecology of these viruses remains patchy. In this study, we aimed to characterize the diversity of giant viruses in Algeria by inoculating 64 environmental samples on various amoeba strains. After isolation by co-culture with nine amoeba supports, flow cytometry and electron microscopy were used to putatively identify viruses. Definitive identification was performed by PCR and sequencing. Mimiviruses, marseilleviruses, faustoviruses and cedratviruses were the main viruses isolated in this study. Moreover, a new virus, which we named fadolivirus, was also isolated and was found to belong to the recent metagenomic descriptions of Klosneuvirinae. Despite the use of 9 amoeba supports for co-culture, most of the isolates were obtained from two amoebas: Acanthamoeba castellanii Neff and Vermamoeba vermiformis CDC 19. Finally, the viruses most frequently isolated were marseilleviruses (55.5%) and Mimiviruses (22.2%). This work shows that the isolation of viruses previously detected by metagenomic analyses can be tedious, but possible. Full article
(This article belongs to the Special Issue Giant Virus Biology and Biodiversity)
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10 pages, 3842 KB  
Article
Kaumoebavirus, a New Virus That Clusters with Faustoviruses and Asfarviridae
by Leena H. Bajrai, Samia Benamar, Esam I. Azhar, Catherine Robert, Anthony Levasseur, Didier Raoult and Bernard La Scola
Viruses 2016, 8(11), 278; https://doi.org/10.3390/v8110278 - 28 Oct 2016
Cited by 72 | Viewed by 9340
Abstract
In this study, we report the isolation of a new giant virus found in sewage water from the southern area of Jeddah (Saudi Arabia), with morphological and genomic resemblance to Faustoviruses. This new giant virus, named Kaumoebavirus, was obtained from co-culture with Vermamoeba [...] Read more.
In this study, we report the isolation of a new giant virus found in sewage water from the southern area of Jeddah (Saudi Arabia), with morphological and genomic resemblance to Faustoviruses. This new giant virus, named Kaumoebavirus, was obtained from co-culture with Vermamoeba vermiformis, an amoeboid protozoa considered to be of special interest to human health and the environment. This new virus has ~250 nm icosahedral capsids and a 350,731 bp DNA genome length. The genome of Kaumoebavirus has a coding density of 86%, corresponding to 465 genes. Most of these genes (59%) are closely related to genes from members of the proposed order Megavirales, and the best matches to its proteins with other members of the Megavirales are Faustoviruses (43%) and Asfarviruses (23%). Unsurprisingly, phylogenetic reconstruction places Kaumoebavirus as a distant relative of Faustoviruses and Asfarviruses. Full article
(This article belongs to the Special Issue Viruses of Protozoa)
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