Search Results (2)

Ninety-five-year-old Doris Held, a great niece of Sigmund Freud, has been convening the Shakespeare Reading Group in Northampton, Massachusetts, my hometown, since she moved here in 2016. In the following essay, which is a personal response to my experience of this group of Shakespearean readers, to Doris Held, and to the work of Shakespeare in general, I attempt to chart the full impact of the Bard’s work on my life and on the world around me. I am neither a scholar nor a historian. In a true sense, I am a bystander Shakespearean, who has received deep reward and benefit from the experience, but it is Doris Held and her group who opened my eyes to the precise nature of this unexamined reward. Doris brought the spirit of the group from Cambridge, Massachusetts, where she had been a dues-paying member for decades of something called the Old Cambridge Shakespeare Association, which itself dates to 1880. My wife Debra and I attended the first meeting in Northampton more than a decade ago, and we have been receiving emails from Doris four times a year ever since. While these communications often induce guilt, they invariably lead to pleasures that I would never want to relinquish. That is a complicated dynamic in my routine, and I try to grapple with its ebb and flow in the pages that follow. Each time I get one, I have a version of the same conversation in my head. Is Doris still doing this? Haven’t they done all the plays by now? All things considered, wouldn’t they—and I—rather be home watching a true crime documentary about Gaby Petito on Netflix? What the hell is William Shakespeare to me anyway? At this point, if I’m honest, Shakespeare is Doris. The experience with this group led me in two directions. One took me back to my now long-ago history with Shakespeare’s work as an actor in college. The other took me via historical research into the prehistory of Doris Held’s previous Shakespeare group in Cambridge, Massachusetts. The two paths gave me a deeper grasp of the influence of his work across the world and on my own life. Full article

The species delimitation of the marine bivalve species complex Aequiyoldia eightsii in South America and Antarctica is complicated by mitochondrial heteroplasmy and amplification bias in molecular barcoding. In this study, we compare different data sources (mitochondrial cytochrome c oxidase subunit I (COI) sequences; nuclear and mitochondrial SNPs). Whilst all the data suggest that populations on either side of the Drake Passage belong to different species, the picture is less clear within Antarctic populations, which harbor three distinct mitochondrial lineages (p-dist ≈ 6%) that coexist in populations and in a subset of individuals with heteroplasmy. Standard barcoding procedures lead to amplification bias favoring either haplotype unpredictably and thus overestimate the species richness with high confidence. However, nuclear SNPs show no differentiation akin to the trans-Drake comparison, suggesting that the Antarctic populations represent a single species. Their distinct haplotypes likely evolved during periods of temporary allopatry, whereas recombination eroded similar differentiation patterns in the nuclear genome after secondary contact. Our study highlights the importance of using multiple data sources and careful quality control measures to avoid bias and increase the accuracy of molecular species delimitation. We recommend an active search for mitochondrial heteroplasmy and haplotype-specific primers for amplification in DNA-barcoding studies. Full article