Myo-inositol-1-phosphate synthase (MIPS, EC 5.5.1.4) plays important roles in plant growth and development, stress responses, and cellular signal transduction.
MIPS genes were found preferably expressed during fiber cell initiation and early fast elongation in upland cotton (
Gossypium hirsutum), however, current understanding of the function and regulatory mechanism of
MIPS genes to involve in cotton fiber cell growth is limited. Here, by genome-wide analysis, we identified four
GhMIPS genes anchoring onto four chromosomes in
G. hirsutum and analyzed their phylogenetic relationship, evolutionary dynamics, gene structure and motif distribution, which indicates that
MIPS genes are highly conserved from prokaryotes to green plants, with further exon-intron structure analysis showing more diverse in
Brassicales plants. Of the four
GhMIPS members, based on the significant accumulated expression of
GhMIPS1D at the early stage of fiber fast elongating development, thereby, the
GhMIPS1D was selected to investigate the function of participating in plant development and cell growth, with ectopic expression in the loss-of-function
Arabidopsis mips1 mutants. The results showed that
GhMIPS1D is a functional gene to fully compensate the abnormal phenotypes of the deformed cotyledon, dwarfed plants, increased inflorescence branches, and reduced primary root lengths in
Arabidopsis mips1 mutants. Furthermore, shortened root cells were recovered and normal root cells were significantly promoted by ectopic expression of
GhMIPS1D in
Arabidopsis mips1 mutant and wild-type plants respectively. These results serve as a foundation for understanding the
MIPS family genes in cotton, and suggest that
GhMIPS1D may function as a positive regulator for plant cell elongation.
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