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Keywords = Coix larchryma-jobi L. var. ma-yuen Stapf

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2 pages, 146 KiB  
Erratum
Novel Antihypertensive Peptides Derived from Adlay (Coix larchryma-jobi L. var. ma-yuen Stapf) Glutelin
by Bin Li, Liansheng Qiao, Lingling Li, Yanling Zhang, Kai Li, Lingzhi Wang and Yanjiang Qiao
Molecules 2017, 22(4), 534; https://doi.org/10.3390/molecules22040534 - 27 Mar 2017
Cited by 13 | Viewed by 3340
11 pages, 1020 KiB  
Article
Novel Antihypertensive Peptides Derived from Adlay (Coix larchryma-jobi L. var. ma-yuen Stapf) Glutelin
by Bin Li, Liansheng Qiao, Lingling Li, Yanling Zhang, Kai Li, Lingzhi Wang and Yanjiang Qiao
Molecules 2017, 22(1), 123; https://doi.org/10.3390/molecules22010123 - 13 Jan 2017
Cited by 33 | Viewed by 7451
Abstract
Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by [...] Read more.
Our previous studies have shown that Coix glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from Coix glutelin. The Coix glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identified using nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC50 = 14.19 μmol·L−1) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensive rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and the effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from Coix glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases. Full article
(This article belongs to the Special Issue Peptide-Based Drugs and Drug Delivery Systems)
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