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In Mediterranean streams and rivers in general, aquatic organisms use a specific habitat for rearing, growing, breeding, and wintering. Multiple studies have focused on this subject, but few for the specific purpose of developing suitability models that feed hydrobiological models for the analysis of flow regimes and the design of environmental flows. Therefore, this study analyzes the habitat preferences of five freshwater species of fish and mussels in the NE of the Iberian Peninsula for that purpose. We use simple decision trees and random forest (RF), a machine learning technique based on the aggregation of multiple decision trees, to develop suitability models that relate the habitat preferences of the five species—separately adults and juveniles—to different attributes of a physical habitat at the meso-scale. Selected attributes are the surface percentage of different levels of depth (0–15 cm, 15–30 cm, …, >120 cm), velocity (0–15 cm/s, 15–30 cm/s, …, >120 cm/s) and abiotic/biotic substrate (e.g., gigalithal, megalithal, detritus, phytal), and absence/presence of refuges (boulder, canopy shading, emerging vegetation, undercut banks, woody debris, roots). The models were developed in order to predict three ranks of habitat suitability: absence, presence and abundance, depending on the mentioned attributes of the mesohabitat analysed. Our study provides quantitative results concerning the correspondence between the presence and abundance of different species and habitat characteristics, confirming qualitative observations stated in previous studies. We proved now that the adult mussels of Unio genus require a minimum of 5% of sand or silt, low velocities, and undercut banks and roots; that Barbus meridionalis habitat changes considerably among seasons; that Salaria fluviatilis needs coarse substrates (megalithal, macrolithal and mesolithal) and velocities above 15 cm/s; and that the adult Squalius laietanus prefers glides and pools with depths above 60 cm and velocities below 45 cm/s, depending on the season; and that Anguilla anguilla prefers intermediate size substrates (macrolithal, mesolithal and microlithal). These results are essential for the modeling of environmental flows in rivers where these species are present. Thus, by analyzing how their physical habitat changes according to the flow regime, one can see whether the available habitat of fish and mussels increases or decreases and predict periods of danger for the species. Full article

In recent years, a remarkable effort has been made to recover the connectivity for fish in the rivers of Catalonia, mainly through the construction of new fish ladders, or river connectors. However, evaluating the effectiveness of new fish ladders is not yet a common practice, although knowing how it works is a key aspect of improving the design of new river connectors in other infrastructures. For this reason, the Catalan Water Agency has launched a study to monitor the effectiveness of several recently built river connectors. A total of five fish ladders were selected in the rivers Anoia, Ripoll, Brugent, Llémena and Borró, placed in four different watersheds (Llobregat, Besós, Ter and Fluvià, respectively). These rivers have a similar hydrology (average river discharge inferior to 1 m³/s), and a similar potential fish assemblage (Barbus haasi or B. meridionalis, Squaliuslaietanus, Anguilla anguilla, and in some cases Salaria fluviatilis). Several exotic species are also present in some of these rivers: Gambusia holbrooki, Lepomis gibbosus, Phoxinus sp., Barbatula sp. During 2021, between two and three monitoring campaigns (spring, summer, fall) were carried out. The methodology used consisted first in the installation of permanent traps at the top exit of ladders to obtain direct estimates of pass rate by species, and on comparative fish sampling on each side of the barrier. Additionally, regular monitoring of several hydraulic variables (water velocity, draft, and elevation) was performed at a selection of internal points on each ladder. Finally, the expected mobility per species was also estimated using the package Fishmove (Radinger, 2013), to compare it with experimental estimations and determine the efficiency of ladders. The results demonstrate the effectiveness of the evaluated ladders, at least for some of the species present. Among native species, barbels (B. haasi and B. meridionalis) showed the highest rates of passage. Some exotic species were also able to occasionally use the ladders. The efficiency of the ladders is mostly high but decreases when there are internal points with excessive speeds, or other failures with respect to the optimal design. Full article