Search Results (2)

The purpose of this study was to identify seminal plasma proteins in Bali bull and their potential as biomarkers of fertility. Semen was collected from 10 bulls aged 5–10 years using an artificial vagina. Fresh semen was then centrifuged (3000× g for 30 min). The supernatant was put into straws and stored in liquid nitrogen. The semen plasma protein concentration was determined using the Bradford method, and the protein was characterized using 1D-SDS-PAGE. Coomassie Brilliant Blue (CBB) was used to color the gel, and the molecular weight of the protein was determined using PM2700. A total of 94 proteins were identified in the seminal plasma of Bali bulls analyzed using LC-MS/MS (liquid chromatography–mass spectrometry). Proteins spermadhesin 1 (SPADH1), C-type natriuretic peptide (NPPC), clusterin (CLU), apoliprotein A-II (APOA2), inositol-3-phosphate synthase 1 (ISYNA1), and sulfhydryl oxidase 1 (QSOX1) were identified as important for fertility in Bos javanicus. These proteins may prove to be important biomarkers of fertility in Bali bulls. These proteins are important for reproductive function, which includes spermatozoa motility, capacitation, and acrosome reactions. This study provides new information about the protein content in seminal plasma in Bali bulls. The LC-MS/MS-based proteome approach that we applied in this study obtained 94 proteins. The identification of these seminal plasma proteins of Bali bulls and their potential as fertility biomarkers may have an impact on the success of future artificial insemination (AI). Full article

This research was carried out on-farm at Malonas village, Central Sulawesi, Indonesia to examine the effect of high level supplementation of by-products formulated for high ME and CP. Feed intake, faecal pH and liveweight gain of Bali bulls was observed. The basal diet was elephant grass, and supplements were rice bran (RB), cassava (C), palm kernel meal (PKM), gliricidia (G) and urea. Fifty Bali bulls (weight 168 ± 4.48 kg) were housed in individual pens for 18 weeks (2 introductory and 16 experimental) and allocated into five treatments: A = elephant grass (EG) ad libitum, B = EG offered at 1%W/d, plus 2.5%W/d mixed RB:G, (1:1), C = EG offered at 1%W/d plus 2.5%W/d mixed RB:PKM(1:1), D = EG offered at 1%W/d plus 2.5%W/d mixed C:G (1:1), E = EG offered at 1%W/d plus 2.5%W/d mixed C+ urea: PKM (1:1). Faecal pH was measured at weeks 3, 9 and 15. Bali bulls fed B, C, D and E, consumed supplement at the rate of 2.15, 2.19, 2.09 and 2.29 %W/d, respectively. Total feed intake was 2.47, 2.98, 2.99, 2.93, 3.13%W/d, liveweight gain was 0.30, 0.57, 0.60, 0.66, 0.69 g/d and faecal pH was 6.93, 6.76, 6.65, 6.45, 6.33 for treatment A, B, C, D, and E, respectively. Supplementation increased significantly (P < 0.05) total feed intake, liveweight gain, but reduced faecal pH. The highest total feed intake and liveweight gain was achieved by treatment E. It is concluded that cassava can be provided at 1.15%W/d in combination with protein supplement for high liveweight gain of Bali bulls. Full article