The culinary medicinal mushroom
Hericium erinaceus holds significant global esteem and has garnered heightened interest within increasingly ageing societies due to its pronounced neuroprotective and anti-neuroinflammatory properties. Within this study, two novel diterpenes, 16-carboxy-13-
epi-neoverrucosane (
1) and Erinacine L (
2); three known xylosyl cyathane diterpenoids, Erinacine A (
3), Erinacine C (
4), and Erinacine F (
5); and four lanostane-type triterpenoids, and three cyclic dipeptides (
10–
12), in addition to orcinol (
13), were isolated from the rice-based cultivation medium of
H. erinaceus. Their structures were determined by NMR, HR-ESI-MS, ECD, and calculated NMR. Compound
1 marks a pioneering discovery as the first verrucosane diterpene originating from basidiomycetes, amplifying the scope of fungal natural product chemistry, and the intricate stereochemistry of Compound
5 has been comprehensively assessed for the first time. Compounds
2–
5 not only showed encouraging neurotrophic activity in rat adrenal pheochromocytoma PC-12 cells, but also significantly inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV2 microglia cell cultures with IC
50 values as low as 5.82 ± 0.18 μM. To elucidate the mechanistic underpinnings of these bioactivities, molecular docking simulation was used to analyze and support the interaction of
1 and
2 with inducible NO synthase (iNOS), respectively. In particular, compound
2, a cyathane-xyloside containing an unconventional hemiacetal moiety, is a compelling candidate for the prevention of neurodegenerative diseases. In summation, this investigation contributes substantively to the panorama of fungal diterpene structural diversity, concurrently furnishing additional empirical substantiation for the role of cyathane diterpenes in the amelioration of neurodegenerative afflictions.
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