Search Results (3)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mainly targets the upper respiratory tract. It gains entry by interacting with the host cell receptor angiotensin-converting enzyme 2 (ACE2) via its heavily glycosylated spike glycoprotein. SARS-CoV-2 can also affect the gastrointestinal tract. Given the significant role of glycosylation in the life cycle of proteins and the multisystem target of SARS-CoV-2, the role of glycosylation in the interaction of S1 with ACE2 in Caco-2 cells was investigated after modulation of their glycosylation patterns using N-butyldeoxynojirimycin (NB-DNJ) and 1-deoxymannojirimycin (dMM), in addition to mutant CHO cells harboring mutations at different stages of glycosylation. The data show a substantial reduction in the interactions between the altered glycosylation forms of S1 and ACE2 in the presence of NB-DNJ, while varied outcomes resulted from dMM treatment. These results highlight the promising effects of NB-DNJ and its potential use as an off-label drug to treat SARS-CoV-2 infections. Full article

In the present study, effect of catanospermine (CST), 1-deoxynojirimycin (DNJ) or 1-deoxymannojirimycin (DMJ) was studied on porcine stable kidney (PS) cells infected with Japanese encephalitis virus (JEV). As both CST and DNJ are potent inhibitors of ER alpha-glucosidases 1 and II, while DMJ is an inhibitor of Golgi mannosidase which removes alpha (1, 2) Man residues from the N-glycan precursor. Treatment of infected cells with CST (200 uM/mL), DNJ (100 uM/mL) or DMJ (200 uM/mL) did not produce much effect on viral gpE epitope presentation within the cells as well as on the cell surface as detected in the immunofluorescence employing monoclonal (MAbs) and polyclonal (PAbs) antibodies. As well the treated (infected) cells showed only a marginal decrease in infectious virus yield along with a slight decrease in haemagglutination activity of the virus that was recorded in comparison to the untreated infected (control) cells and the cells infected with Dengue virus. Immuno-blotting of the separated proteins from infected lysed cells and probed with anti-gpE MAbs also revealed a band corresponding to JEV gpE (MW 53 kDa) both with inhibitor treated and the untreated cells; the reactivity with the former however, was somewhat less intense and prominent in comparison to latter (control untreated) indicating some effect on JEV. The present results indicate that these inhibitors by in large, do not affect maturation and the release of infective JE virions in PS cells. Full article

The efficient transformation of D-glucal to (2R)-hydroxymethyldihydro-pyridinone 5 in seven steps and 35 % overall yield is reported. Dihydropyridone 5 constitutes a versatile chiral building block for the synthesis of various piperidine alkaloids. In this regard, 5 was converted to piperidinol 13 and piperidinone 15, that may be further elaborated for the syntheses of (+)-desoxoprosophylline (1) and deoxymannojirimycin (3) or D-mannolactam (4), respectively. Full article