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Authors = Yana E. Sergeeva ORCID = 0000-0002-6190-8800

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14 pages, 4610 KiB  
Article
Porous Polylactide Microparticles as Effective Fillers for Hydrogels
by Yuriy D. Zagoskin, Yana E. Sergeeva, Yuliya S. Fomina, Daniil V. Sukhinov, Sergey N. Malakhov, Egor O. Osidak, Elena A. Khramtsova, Pavel M. Gotovtsev, Sergei N. Chvalun and Timofei E. Grigoriev
Biomimetics 2023, 8(8), 565; https://doi.org/10.3390/biomimetics8080565 - 23 Nov 2023
Cited by 3 | Viewed by 2180
Abstract
High-strength composite hydrogels based on collagen or chitosan–genipin were obtained via mixing using highly porous polylactide (PLA) microparticles with diameters of 50–75 µm and porosity values of over 98%. The elastic modulus of hydrogels depended on the filler concentration. The modulus increased from [...] Read more.
High-strength composite hydrogels based on collagen or chitosan–genipin were obtained via mixing using highly porous polylactide (PLA) microparticles with diameters of 50–75 µm and porosity values of over 98%. The elastic modulus of hydrogels depended on the filler concentration. The modulus increased from 80 kPa to 400–600 kPa at a concentration of porous particles of 12–15 wt.% and up to 1.8 MPa at a filling of 20–25 wt.% for collagen hydrogels. The elastic modulus of the chitosan–genipin hydrogel increases from 75 kPa to 900 kPa at a fraction of particles of 20 wt.%. These elastic modulus values cover a range of strength properties from connective tissue to cartilage tissue. It is important to note that the increase in strength in this case is accompanied by a decrease in the density of the material, that is, an increase in porosity. PLA particles were loaded with C-phycocyanin and showed an advanced release profile up to 48 h. Thus, composite hydrogels mimic the structure, biomechanics and release of biomolecules in the tissues of a living organism. Full article
(This article belongs to the Special Issue Fabrication of Polymeric Hydrogels)
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14 pages, 10310 KiB  
Article
Chitosan Sponges for Efficient Accumulation and Controlled Release of C-Phycocyanin
by Yana E. Sergeeva, Anastasia A. Zakharevich, Daniil V. Sukhinov, Alexandra I. Koshkalda, Mariya V. Kryukova, Sergey N. Malakhov, Christina G. Antipova, Olga I. Klein, Pavel M. Gotovtsev and Timofei E. Grigoriev
BioTech 2023, 12(3), 55; https://doi.org/10.3390/biotech12030055 - 17 Aug 2023
Cited by 6 | Viewed by 3401
Abstract
The paper proposed a new porous material for wound healing based on chitosan and C-phycocyanin (C-PC). In this work, C-PC was extracted from the cyanobacteria Arthrospira platensis biomass and purified through ammonium sulfate precipitation. The obtained C-PC with a purity index (PI) of [...] Read more.
The paper proposed a new porous material for wound healing based on chitosan and C-phycocyanin (C-PC). In this work, C-PC was extracted from the cyanobacteria Arthrospira platensis biomass and purified through ammonium sulfate precipitation. The obtained C-PC with a purity index (PI) of 3.36 ± 0.24 was loaded into a chitosan sponge from aqueous solutions of various concentrations (250, 500, and 1000 mg/L). According to the FTIR study, chitosan did not form new bonds with C-PC, but acted as a carrier. The encapsulation efficiency value exceeded 90%, and the maximum loading capacity was 172.67 ± 0.47 mg/g. The release of C-PC from the polymer matrix into the saline medium was estimated, and it was found 50% of C-PC was released in the first hour and the maximum concentration was reached in 5–7 h after the sponge immersion. The PI of the released C-PC was 3.79 and 4.43 depending on the concentration of the initial solution. Full article
(This article belongs to the Section Medical Biotechnology)
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