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Authors = Usha Rawat

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13 pages, 1903 KiB  
Article
An Epidemiological Analysis of SARS-CoV-2 Genomic Sequences from Different Regions of India
by Pragya D. Yadav, Dimpal A. Nyayanit, Triparna Majumdar, Savita Patil, Harmanmeet Kaur, Nivedita Gupta, Anita M. Shete, Priyanka Pandit, Abhinendra Kumar, Neeraj Aggarwal, Jitendra Narayan, Neetu Vijay, Usha Kalawat, Attayur P. Sugunan, Ashok Munivenkatappa, Tara Sharma, Sulochna Devi, Tapan Majumdar, Subhash Jaryal, Rupinder Bakshi, Yash Joshi, Rima Sahay, Jayanti Shastri, Mini Singh, Manoj Kumar, Vinita Rawat, Shanta Dutta, Sarita Yadav, Kaveri Krishnasamy, Sharmila Raut, Debasis Biswas, Biswajyoti Borkakoty, Santwana Verma, Sudha Rani, Hirawati Deval, Disha Patel, Jyotirmayee Turuk, Bharti Malhotra, Bashir Fomda, Vijaylakshmi Nag, Amita Jain, Anudita Bhargava, Varsha Potdar, Sarah Cherian, Priya Abraham, Anjani Gopal, Samiran Panda and Balram Bhargavaadd Show full author list remove Hide full author list
Viruses 2021, 13(5), 925; https://doi.org/10.3390/v13050925 - 17 May 2021
Cited by 30 | Viewed by 7152
Abstract
The number of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) cases is increasing in India. This study looks upon the geographic distribution of the virus clades and variants circulating in different parts of India between January and August 2020. The NPS/OPS from representative positive [...] Read more.
The number of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) cases is increasing in India. This study looks upon the geographic distribution of the virus clades and variants circulating in different parts of India between January and August 2020. The NPS/OPS from representative positive cases from different states and union territories in India were collected every month through the VRDLs in the country and analyzed using next-generation sequencing. Epidemiological analysis of the 689 SARS-CoV-2 clinical samples revealed GH and GR to be the predominant clades circulating in different states in India. The northern part of India largely reported the ‘GH’ clade, whereas the southern part reported the ‘GR’, with a few exceptions. These sequences also revealed the presence of single independent mutations—E484Q and N440K—from Maharashtra (first observed in March 2020) and Southern Indian States (first observed in May 2020), respectively. Furthermore, this study indicates that the SARS-CoV-2 variant (VOC, VUI, variant of high consequence and double mutant) was not observed during the early phase of virus transmission (January–August). This increased number of variations observed within a short timeframe across the globe suggests virus evolution, which can be a step towards enhanced host adaptation. Full article
(This article belongs to the Special Issue Genomic Epidemiology of Viral Infections)
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5 pages, 170 KiB  
Short Note
A New Flavonoid Glycoside from Salix denticulata Aerial Parts
by Usha Rawat, Sushma Semwal, Deepak Kumar Semwal, Ruchi Badoni and Amita Bamola
Molbank 2009, 2009(3), M622; https://doi.org/10.3390/M622 - 23 Sep 2009
Cited by 4 | Viewed by 8597
Abstract
Abstract: A new flavonoid glycoside (1) has been isolated from the aerial parts of Salix denticulata (Salicaceae) together with five known compounds, β-sitosterol, 2,6-dihydroxy- 4-methoxy acetophenone, eugenol-1-O-β-D-glucopyranoside, 1-O-β-D-(3’-benzoyl) salicyl alcohol and luteolin-7-O-β-D-glucopyranosyl-(1-6)-glucopyranoside. The structure of 1 was [...] Read more.
Abstract: A new flavonoid glycoside (1) has been isolated from the aerial parts of Salix denticulata (Salicaceae) together with five known compounds, β-sitosterol, 2,6-dihydroxy- 4-methoxy acetophenone, eugenol-1-O-β-D-glucopyranoside, 1-O-β-D-(3’-benzoyl) salicyl alcohol and luteolin-7-O-β-D-glucopyranosyl-(1-6)-glucopyranoside. The structure of 1 was elucidated as 2’,5-dihydroxy-3’-methoxyflavone-7-O-β-D-glucopyranoside by means of chemical and spectral data including 2D NMR studies. Full article
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5 pages, 189 KiB  
Short Note
Further Aporphine Alkaloids from Phoebe lanceolata
by Deepak K. Semwal, Usha Rawat and G. J. P. Singh
Molbank 2008, 2008(3), M581; https://doi.org/10.3390/M581 - 23 Nov 2008
Cited by 3 | Viewed by 5531
Abstract
Stem bark of Phoebe lanceolata was extracted with ethanol and fractionated with ethyl acetate yielded soluble and insoluble fractions. Ethyl acetate insoluble fraction was subjected to column chromatography afforded two oxalyl-fused didehydroaporphine alkaloids, N-6/C-7 oxalyl-fused 2,9-dihydroxy-1,10-dimethoxy 6a,7-didehydroaporphine and N-6/C-7 oxalyl-fused 1,2,9,10-tetramethoxy 6a,7-didehydroaporphine along [...] Read more.
Stem bark of Phoebe lanceolata was extracted with ethanol and fractionated with ethyl acetate yielded soluble and insoluble fractions. Ethyl acetate insoluble fraction was subjected to column chromatography afforded two oxalyl-fused didehydroaporphine alkaloids, N-6/C-7 oxalyl-fused 2,9-dihydroxy-1,10-dimethoxy 6a,7-didehydroaporphine and N-6/C-7 oxalyl-fused 1,2,9,10-tetramethoxy 6a,7-didehydroaporphine along with well known β-sitosterol and β-sitosterol glucoside. The structures of isolated compounds were elucidated by chemical and spectral analysis. Full article
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