Search Results (12)

Aluminum oxide clusters (AlOCs) possess high surface areas and customizable pore structures, making them applicable in the field of environmental remediation. However, their practical use is hindered by stability issues, aggregation tendencies, and recycling challenges. This study presents an in -situ synthesis of AlOCs on cellulose using a solvent thermal method. The resulting adsorbent’s structural and property profiles were thoroughly characterized using multiple analytical techniques. Batch adsorption experiments were performed to assess the adsorbent’s capacity and kinetics in removing selected dyes from aqueous solutions. Additionally, both real-environment simulation and regeneration experiments have been conducted to thoroughly assess the adsorbent’s reliability, stability, and practical applicability. The aim was to engineer an effective and recyclable adsorbent specifically tailored for dye-contaminated wastewater treatment. Full article

As a specific task for unmanned tracked vehicles, leader-following imposes high-precision requirements on the vehicle’s motion control, especially the steering control. However, due to characteristics such as the frequent changes in off-road terrain and steering resistance coefficients, controlling tracked vehicles poses significant challenges, making it difficult to achieve stable and precise leader-following. This paper decouples the leader-following control into speed and curvature control to address such issues. It utilizes model reference adaptive control to establish reference models for the speed and curvature subsystems and designs corresponding parameter adaptive control laws. This control method enables the actual vehicle speed and curvature to effectively track the response of the reference model, thereby addressing the impact of frequent changes in the steering resistance coefficient. Furthermore, this paper demonstrates significant improvements in leader-following performance through a series of simulations and experiments. Compared with the traditional PID control method, the results shows that the maximum following distance has been reduced by at least approximately 12% (ensuring the ability to keep up with the leader), the braking distance has effectively decreased by 22% (ensuring a safe distance in an emergency braking scenario and improving energy recovery), the curvature tracking accuracy has improved by at least 11% (improving steering performance), and the speed tracking accuracy has increased by at least 3.5% (improving following performance). Full article

Carbon nanotube-based derivatives have attracted considerable research interest due to their unique structure and fascinating physicochemical properties. However, the controlled growth mechanism of these derivatives remains unclear, and the synthesis efficiency is low. Herein, we proposed a defect-induced strategy for the efficient heteroepitaxial growth of single-wall carbon nanotubes (SWCNTs)@hexagonal boron nitride (h-BN) films. Air plasma treatment was first performed to generate defects on the wall of SWCNTs. Then, atmospheric pressure chemical vapor deposition was conducted to grow h-BN on the surface of SWCNTs. Controlled experiments combined with first-principles calculations revealed that the induced defects on the wall of SWCNTs function as nucleation sites for the efficient heteroepitaxial growth of h-BN. Full article

(1) Background: The aim of this study was to produce in-house ELISAs which can be used to determine SARS-CoV-2-specific antibody levels directed against the spike protein (S), the S1 subunit of S and the receptor binding domain (RBD) of S in SARS-CoV-2 vaccinated and infected humans. (2) Methods: Three in-house ELISAs were developed by using recombinant proteins of SARS-CoV-2, namely the S, S1 and RBD proteins. Specificity and sensitivity evaluations of these tests were performed using sera from SARS-CoV-2-infected (n = 70) and SARS-CoV-2-vaccinated (n = 222; CoronaVac vaccine) humans in Istanbul, Turkey. The analyses for the presence of SARS-CoV-2-specific antibodies were performed using the in-house ELISAs, a commercial ELISA (Abbott) and a commercial surrogate virus neutralization test (sVNT). We also analyzed archival human sera (n = 50) collected before the emergence of COVID-19 cases in Turkey. (3) Results: The sensitivity of the in-house S, S1 and RBD ELISAs was found to be 88.44, 90.17 and 95.38%, while the specificity was 72.27, 89.08 and 89.92%, respectively, when compared to the commercial SARS-CoV-2 antibody test kit. The area under curve (AUC) values were 0.777 for the in-house S ELISA, 0.926 for the S1 ELISA, and 0.959 for the RBD ELISA. The kappa values were 0.62, 0.79 and 0.86 for the S, S1 and RBD ELISAs, respectively. (4) Conclusions: The in-house S1 and RBD ELISAs developed in this study have acceptable performance characteristics in terms of sensitivity, specificity, AUC and kappa values. In particular, the RBD ELISA seems viable to determine SARS-CoV-2-specific antibody levels, both in infected and vaccinated people, and help mitigate SARS-CoV-2 outbreaks and spread. Full article

To investigate the influence of coal pillar width on the stress variation of narrow coal pillar (NCP) in the gob-side tunnel in an inclined thick coal seam, theoretical analysis, numerical modeling, and field monitoring are performed to determine the optimal width of the narrow coal pillars in inclined coal seams. The mechanical characteristics of the NCP for varying widths were investigated. Furthermore, vertical and horizontal stress were calculated for various widths of the NCP. The results revealed that with the rise in the width, the vertical stress initially increased dramatically and then stabilized, whereas the mean horizontal stress increased gradually. The mathematical relation between stress and NCP widths was represented by two fitting equations. The evolution process of the plastic zone in the NCP under various widths and the damage form of various widths were obtained; that is, when the width was small, the position of the roadway near the shoulder corner of NCP was inclined to the top of NCP. The field monitoring data revealed that the optimum NCP width was 4 m. This NCP width could stabilize the roadway and improve the loss prevention of the NCP at the gob-side tunnel of similar mines. Full article

Duck enteritis virus (DEV) and Pasteurella multocida, the causative agent of duck plague and fowl cholera, are acute contagious diseases and leading causes of morbidity and mortality in duck. The NHEJ-CRISPR/Cas9-mediated gene editing strategy, accompanied with the Cre–Lox system, have been employed in the present study to show that two new sites at UL55-LORF11 and UL44-44.5 loci in the genome of the attenuated Jansen strain of DEV can be used for the stable expression of the outer membrane protein H (ompH) gene of P. multocida that could be used as a bivalent vaccine candidate with the potential of protecting ducks simultaneously against major viral and bacterial pathogens. The two recombinant viruses, DEV-OmpH-V5-UL55-LORF11 and DEV-OmpH-V5-UL44-44.5, with the insertion of ompH-V5 gene at the UL55-LORF11 and UL44-44.5 loci respectively, showed similar growth kinetics and plaque size, compared to the wildtype virus, confirming that the insertion of the foreign gene into these did not have any detrimental effects on DEV. This is the first time the CRISPR/Cas9 system has been applied to insert a highly immunogenic gene from bacteria into the DEV genome rapidly and efficiently. This approach offers an efficient way to introduce other antigens into the DEV genome for multivalent vector. Full article

Improving the immunogenicity and protective efficacy of vaccines is critical to reducing disease impacts. One strategy used to enhance the immunogenicity of vaccines is the selective delivery of protective antigens to the antigen presenting cells (APCs). In this study, we have developed a targeted antigen delivery vaccine (TADV) system by recombinantly fusing the ectodomain of hemagglutinin (HA) antigen of H9N2 influenza A virus to single chain fragment variable (scFv) antibodies specific for the receptors expressed on chicken APCs; Dec205 and CD11c. Vaccination of chickens with TADV containing recombinant H9HA Foldon-Dec205 scFv or H9HA Foldon-CD11c scFv proteins elicited faster (as early as day 6 post primary vaccination) and higher anti-H9HA IgM and IgY, haemagglutination inhibition, and virus neutralisation antibodies compared to the untargeted H9HA protein. Comparatively, CD11c scFv conjugated H9HA protein showed higher immunogenic potency compared to Dec205 scFv conjugated H9HA protein. The higher immune potentiating ability of CD11c scFv was also reflected in ex-vivo chicken splenocyte stimulation assay, whereby H9HA Foldon-CD11c scFv induced higher levels of cytokines (IFNγ, IL6, IL1β, and IL4) compared to H9HA Foldon-Dec205 scFv. Overall, the results conclude that TADV could be a better alternative to the currently available inactivated virus vaccines. Full article

Graphene- and carbon nanotube (CNT)-based inks have been printed on relevant heat sink materials by Aerosol jet. The thickness of the layers varied between ~100 and ~1.500 nm. The inks’ viscosity ranged from <20 up to 600 cps at a solid content between 0.18 and 3% and wide particle sizes from 5 nm up to 5 µm. The printed layers could be interesting for rather high-power and high-temperature applications including thermal heat spreaders, resistive heaters, high-current carrying interconnectors, temperature sensors and ordnance fuze technology. Full article

The sensitivity enhancement of the weakly tilted fiber Bragg grating (WTFBG) integrated with black phosphorus (BP) was investigated via numerical simulations and experimental demonstrations. BP nanosheets were deposited twice on the cylindrical WTFBG surface using the in situ layer-by-layer (i-LbL) deposition technique. The resonance intensity of the deepest cladding mode located around 1552 nm of WTFBG had a 9.2 dB decrease after the BP deposition process. This allows for the application of the intensity-modulated refractive index (RI) sensor. The sensing platform was implemented on the use of the BP integrated with WTFBG (BP-WTFBG). The refractometric sensing was achieved with the sensitivity enhancement of the resonance intensity modulation of the deepest cladding mode for the BP-WTFBG. The sensitivities were 137.6 dB/RIU and 75.6 dB/RIU in the RI region of 1.33–1.35 and 1.35–1.38, respectively. This platform shows great potential applications for biochemical sensing because of its highly sensitive RI sensing ability around the biochemical sensing window. Full article

Passive immunisation with neutralising antibodies can be a potent therapeutic strategy if used pre- or post-exposure to a variety of pathogens. Herein, we investigated whether recombinant monoclonal antibodies (mAbs) could be used to protect chickens against avian influenza. Avian influenza viruses impose a significant economic burden on the poultry industry and pose a zoonotic infection risk for public health worldwide. Traditional control measures including vaccination do not provide rapid protection from disease, highlighting the need for alternative disease mitigation measures. In this study, previously generated neutralizing anti-H9N2 virus monoclonal antibodies were converted to single-chain variable fragment antibodies (scFvs). These recombinant scFv antibodies were produced in insect cell cultures and the preparations retained neutralization capacity against an H9N2 virus in vitro. To evaluate recombinant scFv antibody efficacy in vivo, chickens were passively immunized with scFvs one day before, and for seven days after virus challenge. Groups receiving scFv treatment showed partial virus load reductions measured by plaque assays and decreased disease manifestation. These results indicate that antibody therapy could reduce clinical disease and shedding of avian influenza virus in infected chicken flocks. Full article

Avian influenza viruses (AIVs) are highly contagious and have caused huge economical loss to the poultry industry. AIV vaccines remain one of the most effective methods of controlling this disease. Turkey herpesvirus (HVT) is a commonly used live attenuated vaccine against Marek’s disease; it has also been used as a viral vector for recombinant AIV vaccine development. The clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system is a gene editing tool which, in vaccinology, has facilitated the development of recombinant DNA viral-vectored vaccines. Here, we utilize homology-directed repair (HDR) for the generation of a HVT–H7N9 HA bivalent vaccine; a H7N9 HA expression cassette was inserted into the intergenic region between UL45 and UL46 of HVT. To optimize the selection efficiency of our bivalent vaccine, we combined CRISPR/Cas9 with erythrocyte binding to rapidly generate recombinant HVT–H7HA candidate vaccines. Full article

Duck-targeted vaccines to protect against avian influenza are critically needed to aid in influenza disease control efforts in regions where ducks are endemic for highly pathogenic avian influenza (HPAI). Duck enteritis virus (DEV) is a promising candidate viral vector for development of vaccines targeting ducks, owing to its large genome and narrow host range. The clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system is a versatile gene-editing tool that has proven beneficial for gene modification and construction of recombinant DNA viral vectored vaccines. Currently, there are two commonly used methods for gene insertion: non-homologous end-joining (NHEJ) and homology-directed repair (HDR). Owing to its advantages in efficiency and independence from molecular requirements of the homologous arms, we utilized NHEJ-dependent CRISPR/Cas9 to insert the influenza hemagglutinin (HA) antigen expression cassette into the DEV genome. The insert was initially tagged with reporter green fluorescence protein (GFP), and a Cre-Lox system was later used to remove the GFP gene insert. Furthermore, a universal donor plasmid system was established by introducing double bait sequences that were independent of the viral genome. In summary, we provide proof of principle for generating recombinant DEV viral vectored vaccines against the influenza virus using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system. Full article