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Authors = Paulo G. de Sousa Júnior ORCID = 0000-0003-0105-1515

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14 pages, 5366 KiB  
Article
Investigation of Mn2+-Doped Stearic-Acid Through XRD, Raman, and FT-IR, and Thermal Studies
by Rodrigo M. Rocha, Marinaldo V. de Souza Junior, Luiz F. L. Silva, Paulo T. C. Freire, Gardênia S. Pinheiro, Waldomiro Paschoal, Francisco F. de Sousa and Sanclayton G. C. Moreira
Quantum Beam Sci. 2025, 9(1), 8; https://doi.org/10.3390/qubs9010008 - 1 Mar 2025
Viewed by 1217
Abstract
In this research, we investigated the influence of Mn2+ ions on the packing in stearic acid (SA) crystals through the use of Raman spectroscopy, X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy. The crystals investigated were obtained utilizing the slow evaporation [...] Read more.
In this research, we investigated the influence of Mn2+ ions on the packing in stearic acid (SA) crystals through the use of Raman spectroscopy, X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy. The crystals investigated were obtained utilizing the slow evaporation methodology in a hexane solution under varying manganese (Mn) concentrations sourced from MnSO4 5H2O (0.5, 1.0, 1.5, 2.0, 4.0, and 6.0%). XRD studies indicated that all SA crystals were grown in the Bm form (monoclinic), favoring the gauche conformation in molecular packing. Additionally, crystalline lattice modifications were observed through Raman spectral changes in the low-vibrational energy region. Variations in the intensities and Raman shifts in two lattice vibrational modes, centered at approximately 59 and 70 cm−1, revealed that two types of hydrogen bonds are distinctly affected within the crystalline lattice. Furthermore, the unit cell parameters (a, b, c, and β) were determined via Rietveld refinement, and their behavior was analyzed as a function of Mn concentration. The results indicated that Mn2+ ions exert a strain and deformation effect on the unit cell. Lastly, differential scanning calorimetry (DSC) was employed to evaluate the thermal stability of the Bm form of SA crystals. Full article
(This article belongs to the Section Engineering and Structural Materials)
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37 pages, 7084 KiB  
Review
Lipase from Yarrowia lipolytica: Prospects as an Industrial Biocatalyst for Biotechnological Applications
by Jessica Lopes da Silva, Misael Bessa Sales, Viviane de Castro Bizerra, Millena Mara Rabelo Nobre, Ana Kátia de Sousa Braz, Patrick da Silva Sousa, Antônio L. G. Cavalcante, Rafael L. F. Melo, Paulo Gonçalves De Sousa Junior, Francisco S. Neto, Aluísio Marques da Fonseca and José Cleiton Sousa dos Santos
Fermentation 2023, 9(7), 581; https://doi.org/10.3390/fermentation9070581 - 21 Jun 2023
Cited by 31 | Viewed by 6036
Abstract
This paper aims to present the advances related to the biotechnological application of lipases Y. lipolytica, presenting their properties and more efficient ways to use them in different industrial applications. Waste treatment and bioremediation highlight recent studies and advances and the interest [...] Read more.
This paper aims to present the advances related to the biotechnological application of lipases Y. lipolytica, presenting their properties and more efficient ways to use them in different industrial applications. Waste treatment and bioremediation highlight recent studies and advances and the interest in large-scale applications in the food sector and biofuel production. The USA and China, two major world powers in industy, are of utmost importance in the search for the improvement in the development and properties of a controlled system for the large-scale production of a significant number of applications of lipase from Y. lipolytica. Full article
(This article belongs to the Special Issue Yarrowia lipolytica: A Beneficial Yeast as a Biofactory for Biotechnological Applications)
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28 pages, 2601 KiB  
Review
The Chemistry and Applications of Metal–Organic Frameworks (MOFs) as Industrial Enzyme Immobilization Systems
by Allison R. M. Silva, Jeferson Y. N. H. Alexandre, José E. S. Souza, José G. Lima Neto, Paulo G. de Sousa Júnior, Maria V. P. Rocha and José C. S. dos Santos
Molecules 2022, 27(14), 4529; https://doi.org/10.3390/molecules27144529 - 15 Jul 2022
Cited by 102 | Viewed by 9365
Abstract
Enzymatic biocatalysis is a sustainable technology. Enzymes are versatile and highly efficient biocatalysts, and have been widely employed due to their biodegradable nature. However, because the three-dimensional structure of these enzymes is predominantly maintained by weaker non-covalent interactions, external conditions, such as temperature [...] Read more.
Enzymatic biocatalysis is a sustainable technology. Enzymes are versatile and highly efficient biocatalysts, and have been widely employed due to their biodegradable nature. However, because the three-dimensional structure of these enzymes is predominantly maintained by weaker non-covalent interactions, external conditions, such as temperature and pH variations, as well as the presence of chemical compounds, can modify or even neutralize their biological activity. The enablement of this category of processes is the result of the several advances in the areas of molecular biology and biotechnology achieved over the past two decades. In this scenario, metal–organic frameworks (MOFs) are highlighted as efficient supports for enzyme immobilization. They can be used to ‘house’ a specific enzyme, providing it with protection from environmental influences. This review discusses MOFs as structures; emphasizes their synthesis strategies, properties, and applications; explores the existing methods of using immobilization processes of various enzymes; and lists their possible chemical modifications and combinations with other compounds to formulate the ideal supports for a given application. Full article
(This article belongs to the Section Applied Chemistry)
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