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Authors = Monique J. Farrell

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10 pages, 3088 KiB  
Article
Colorimetric and Label-Free Optical Detection of Pb2+ Ions via Colloidal Gold Nanoparticles
by Jasmin A. Flowers, Monique J. Farrell, Gugu Rutherford and Aswini K. Pradhan
Biosensors 2023, 13(8), 819; https://doi.org/10.3390/bios13080819 - 15 Aug 2023
Cited by 6 | Viewed by 2458
Abstract
The detection of the lead heavy metal (Pb) in water is crucial in many chemical processes, as it is associated with serious health hazards. Here, we report the selective and precise colorimetric detection of Pb2+ ions in water, exploiting the aggregation and [...] Read more.
The detection of the lead heavy metal (Pb) in water is crucial in many chemical processes, as it is associated with serious health hazards. Here, we report the selective and precise colorimetric detection of Pb2+ ions in water, exploiting the aggregation and self-assembly mechanisms of glutathione (GSH)-functionalized gold nanoparticles (GNPs). The carboxyl functional groups are able to create coordination complexes with Pb2+, inducing aggregation amongst the GSH-GNPs in the presence of Pb2+ due to the chelation of the GSH ligands. The resulting aggregation amongst the GSH-GNPs in the presence of Pb2+ increases the aggregate size depending on the available Pb2+ ions, affecting the plasmonic coupling. This causes a substantial shift in the plasmon wavelength to a longer wavelength side with increasing Pb2+ concentration, resulting in a red-to-blue colorimetric or visual change, enabling the instant determination of lead content in water. Full article
(This article belongs to the Section Optical and Photonic Biosensors)
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11 pages, 3837 KiB  
Article
CdSe-ZnO Core–Shell Quantum Dots for Protein Detection: A Potential Sensing Platform
by Erin A. Jenrette, Monique J. Farrell, Jasmin A. Flowers and Aswini K. Pradhan
Nanomanufacturing 2021, 1(1), 3-13; https://doi.org/10.3390/nanomanufacturing1010002 - 9 Apr 2021
Cited by 5 | Viewed by 4359
Abstract
A highly sensitive biosensing platform comprised of CdSe-ZnO core–shell nanostructures for targeted applications in protein detection is demonstrated. This innovative technique uses a microwave-assisted thermal decomposition method to produce a rapid, less hazardous, and user-friendly procedure to synthesize a semiconductor core surrounded by [...] Read more.
A highly sensitive biosensing platform comprised of CdSe-ZnO core–shell nanostructures for targeted applications in protein detection is demonstrated. This innovative technique uses a microwave-assisted thermal decomposition method to produce a rapid, less hazardous, and user-friendly procedure to synthesize a semiconductor core surrounded by nanometer-thick metal oxide shells. The benefit of using a metal oxide shell includes mitigating the toxicity of the CdSe core, thus increasing its biocompatibility and minimizing its photochemical corrosion and oxidation. We present a simple one-pot microwave-assisted protocol for the formation of CdSe-ZnO core–shell quantum dots (QDs). These QDs optimize the recognition limit of bovine serum albumin (BSA) protein through a spectral signal at a considerably low concentration (2.5 × 10−6 M), thus demonstrating its potential to become a highly effective surface-plasmon-enhanced Raman spectroscopy (SERS)-like sensing platform. We report a QD material that can mimic a strong SERS-like behavior due to charge transfer affecting the local electric field. Full article
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12 pages, 6861 KiB  
Article
Optical Detection of Denatured Ferritin Protein via Plasmonic Gold Nanoparticles Exposure through Aminosilane Solution
by Monique J. Farrell, Robert J. Reaume, Erin A. Jenrette, Jasmine Flowers, Kevin C. Santiago, Kyo D. Song and Aswini K. Pradhan
Nanomaterials 2019, 9(10), 1417; https://doi.org/10.3390/nano9101417 - 4 Oct 2019
Cited by 2 | Viewed by 4045
Abstract
The presence of denatured proteins within a therapeutic drug product can create a series of serious adverse effects, such as mild irritation, immunogenicity, anaphylaxis, or instant death to a patient. The detection of protein degradation is complicated and expensive due to current methods [...] Read more.
The presence of denatured proteins within a therapeutic drug product can create a series of serious adverse effects, such as mild irritation, immunogenicity, anaphylaxis, or instant death to a patient. The detection of protein degradation is complicated and expensive due to current methods associated with expensive instrumentation, reagents, and processing time. We have demonstrated here a platform for visual biosensing of denatured proteins that is fast, low cost, sensitive, and user friendly by exploiting the plasmonic properties of noble metal nanoparticles. In this study we have exposed artificially heat stressed ferritin and gold nanoparticles to 3-aminopropyl triethoxysilane, which degrades the protein by showing a systematic blue shift in the absorbance spectra of the gold nanoparticle/ferritin and aminosilane solution. This blue shift in absorbance produces a detectable visual color transition from a blue color to a purple hue. By studying the Raman spectroscopy of the gold nanoparticle/ferritin and aminosilane solution, the extent of ferritin degradation was quantified. The degradation of ferritin was again confirmed using dynamic light scattering and was attributed to the aggregation of the ferritin due to accelerated heat stress. We have successfully demonstrated a proof of concept for visually detecting ferritin from horse spleen that has experienced various levels of degradation, including due to heat stress. Full article
(This article belongs to the Special Issue Gold Nanoparticle-Based Biosensors)
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