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Authors = Maruay Pimprasert

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13 pages, 4925 KiB  
Article
Fertility and Insemination Characteristics of Sperm Storage Tubules in Old Thai-Native Hens
by Theerapat Kheawkanha, Vibuntita Chankitisakul, Maruay Pimprasert, Wuttigrai Boonkum and Thevin Vongpralub
Animals 2024, 14(5), 694; https://doi.org/10.3390/ani14050694 - 23 Feb 2024
Cited by 2 | Viewed by 2525
Abstract
We aimed to evaluate the effects of sperm concentration (150–250 × 106 spz/dose) and insemination frequency (once, twice, and thrice weekly) on fertility and sperm storage tubule (SST) characteristics. The SSTs were classified into five categories: namely, SSTs having an unscorable (SST1), [...] Read more.
We aimed to evaluate the effects of sperm concentration (150–250 × 106 spz/dose) and insemination frequency (once, twice, and thrice weekly) on fertility and sperm storage tubule (SST) characteristics. The SSTs were classified into five categories: namely, SSTs having an unscorable (SST1), empty (SST2), low (SST3), medium (SST4), and high (SST5) sperm count after insemination. The results showed that only insemination frequency affected the fertility rate (p < 0.05). The highest fertility was found in the thrice-weekly insemination group; however, this rate was not significantly different from that for the twice-weekly insemination group, except on day 7, while the once-weekly insemination group showed the lowest fertility rate (p < 0.05) from day four onward. On day 1, the SST characteristics showed no differences among the various insemination frequencies. On day 4, the SST2 and SST3 categories increased in the once-weekly insemination group (p < 0.05), while the SST4 and SST5 categories decreased compared to the twice- and thrice-weekly insemination groups (p < 0.05). On day 7, only the thrice-weekly insemination group maintained a level of SST5 category tubules like that measured on day 1 (p > 0.05). In summary, the insemination dose of 150 × 106 sperm was enough for fertilization, and thrice-weekly insemination was the appropriate frequency in old Thai native hens for maintaining a high sperm density in the SSTs throughout the week. Full article
(This article belongs to the Special Issue Advances in Animal Fertility Preservation—Second Edition)
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11 pages, 4221 KiB  
Article
Influence of Semen Collection Frequency and Seasonal Variations on Fresh and Frozen Semen Quality in Thai Native Roosters
by Maruay Pimprasert, Theerapat Kheawkanha, Wuttigrai Boonkum and Vibuntita Chankitisakul
Animals 2023, 13(4), 573; https://doi.org/10.3390/ani13040573 - 6 Feb 2023
Cited by 19 | Viewed by 12789
Abstract
This study aimed to determine the effects of the frequency of semen collection (once, twice, and thrice weekly) and seasonal variations on the fresh and frozen semen quality of Thai native roosters throughout the year. Data on temperature and humidity were collected and [...] Read more.
This study aimed to determine the effects of the frequency of semen collection (once, twice, and thrice weekly) and seasonal variations on the fresh and frozen semen quality of Thai native roosters throughout the year. Data on temperature and humidity were collected and used to calculate the temperature–humidity index (THI). The average temperature and THI were lower in the winter than in the rainy season and the summer (p < 0.05). In contrast, the average relative humidity was not different among the seasons but was higher in the rainy season (p > 0.05). None of the fresh or frozen semen quality parameters were influenced by the frequency of semen collection, but the season did have an effect. The highest sperm concentration was obtained in the winter (p < 0.05). In contrast, the lowest sperm concentration was found during the rainy season, which presented the highest humidity. Regarding the frozen semen quality, the highest malondialdehyde concentration and the lowest motility were found in the summer (p < 0.05). In conclusion, semen collection can be conducted thrice per week for a consecutive year without affecting semen quality while maximizing sperm production. However, the highest sperm production was obtained in the winter, which is also a suitable season for producing semen for cryopreservation. Full article
(This article belongs to the Section Animal Reproduction)
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