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The hygienic practices of mothers during complementary food feeding are crucial in the protection of vulnerable infants and children aged 6–24 months from childhood communicable diseases such as diarrhea and malnutrition. However, sufficient evidence on the hygienic practices of mothers during complementary food feeding and their associated factors is limited. Thus, this study is aimed at assessing the levels of complementary feeding hygiene practice and its associated factors among mothers of children aged 6–24 months in the Tegedie District, Northwest Ethiopia. Method: A community-based cross-sectional study was conducted from 17 March to 17 April 2021, among 576 mothers with children aged 6–24 months in the Tegedie District, Northwest Ethiopia. A multistage sampling technique was used to select the study participants. Data were collected using a pretested structured questionnaire, then entered into Epi Data version 4.6 and exported to SPSS version 23 for analysis. Bivariable and multivariable logistic regressions were used to identify factors associated with outcome variables, and variables with p-values of <0.05 in the final models were considered statistically significant. Result: The prevalence of hygienic practice during complementary food feeding of their children aged 6–24 months was 33.6%, with 95% (CI 29.7% to 37.6%) of them having good practice. Living in an urban area (AOR = 7.02, 95% CI: (4.14, 11.88)), the presence of a handwashing facility near the latrine (AOR = 3.02, 95% CI: (1.18, 7.70)), the presence of a separate area to store raw and cooked foods (AOR = 5.87, 95% CI: (2.84, 12.13)), and the presence of a three-compartment dish washing system (AOR = 5.70, 95%,CI: (3.41, 9.54)) were predictors of practicing good hygienic complementary food feeding. The prevalence of good hygienic practices during complementary feeding among mothers was low. The district health office and health extension workers should work to improve maternal hygienic practices during complementary feeding. Full article