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Authors = Huw Colin-York ORCID = 0000-0002-6585-3237

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13 pages, 946 KiB  
Review
Quantitative Methodologies to Dissect Immune Cell Mechanobiology
by Veronika Pfannenstill, Aurélien Barbotin, Huw Colin-York and Marco Fritzsche
Cells 2021, 10(4), 851; https://doi.org/10.3390/cells10040851 - 9 Apr 2021
Cited by 2 | Viewed by 5236
Abstract
Mechanobiology seeks to understand how cells integrate their biomechanics into their function and behavior. Unravelling the mechanisms underlying these mechanobiological processes is particularly important for immune cells in the context of the dynamic and complex tissue microenvironment. However, it remains largely unknown how [...] Read more.
Mechanobiology seeks to understand how cells integrate their biomechanics into their function and behavior. Unravelling the mechanisms underlying these mechanobiological processes is particularly important for immune cells in the context of the dynamic and complex tissue microenvironment. However, it remains largely unknown how cellular mechanical force generation and mechanical properties are regulated and integrated by immune cells, primarily due to a profound lack of technologies with sufficient sensitivity to quantify immune cell mechanics. In this review, we discuss the biological significance of mechanics for immune cells across length and time scales, and highlight several experimental methodologies for quantifying the mechanics of immune cells. Finally, we discuss the importance of quantifying the appropriate mechanical readout to accelerate insights into the mechanobiology of the immune response. Full article
(This article belongs to the Special Issue Molecular Mechanisms of Early T Cell Signaling)
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19 pages, 3570 KiB  
Article
Exploring the Potential of Airyscan Microscopy for Live Cell Imaging
by Kseniya Korobchevskaya, B. Christoffer Lagerholm, Huw Colin-York and Marco Fritzsche
Photonics 2017, 4(3), 41; https://doi.org/10.3390/photonics4030041 - 7 Jul 2017
Cited by 67 | Viewed by 29977
Abstract
Biological research increasingly demands the use of non-invasive and ultra-sensitive imaging techniques. The Airyscan technology was recently developed to bridge the gap between conventional confocal and super-resolution microscopy. This technique combines confocal imaging with a 0.2 Airy Unit pinhole, deconvolution and the pixel-reassignment [...] Read more.
Biological research increasingly demands the use of non-invasive and ultra-sensitive imaging techniques. The Airyscan technology was recently developed to bridge the gap between conventional confocal and super-resolution microscopy. This technique combines confocal imaging with a 0.2 Airy Unit pinhole, deconvolution and the pixel-reassignment principle in order to enhance both the spatial resolution and signal-to-noise-ratio without increasing the excitation power and acquisition time. Here, we present a detailed study evaluating the performance of Airyscan as compared to confocal microscopy by imaging a variety of reference samples and biological specimens with different acquisition and processing parameters. We found that the processed Airyscan images at default deconvolution settings have a spatial resolution similar to that of conventional confocal imaging with a pinhole setting of 0.2 Airy Units, but with a significantly improved signal-to-noise-ratio. Further gains in the spatial resolution could be achieved by the use of enhanced deconvolution filter settings, but at a steady loss in the signal-to-noise ratio, which at more extreme settings resulted in significant data loss and image distortion. Full article
(This article belongs to the Special Issue Superresolution Optical Microscopy)
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