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In indoor housing systems, repetitive contact with the stall partitions may reflect issues between the housing environment and the cow, by reducing the quality of rest and hindering ease of movement. The objective of this study was to validate the ability of a contact mat (CM) system to monitor cow contact with stall dividers and neck rail when compared to video observation. Eleven lactating cows were monitored using video recording and with the CM system for 4 h/d for 4 consecutive days. CM were affixed to the stall dividers and neck rail to record the frequency of cow contact per second. Two observers recorded the frequency of cow contact against the stall partitions per second using three criteria: cow contact with the stall dividers or neck rail regardless of CM contact (Total Rail Contact), cow contact with the CM regardless of placement or force (Total CM Contact), and cow contact with the CM through proper placement and force (Effective CM Contact). The correlation strength used to measure agreement between video observation and CM system for cow ranking based on degree of contact varied from moderate to very high (dividers: Total Rail Contact: r_s = 0.68, Total CM Contact r_s = 0.90, Effective CM Contact r_s = 0.90; neck rail: Total Rail Contact: r_s = 0.71, Total CM Contact r_s = 0.66, Effective CM Contact r_s = 0.58). CM can be used to accurately rank cows based on their frequency of contact with the stall dividers. This can be used to identify individual cows that need intervention for stall comfort risk factors, or to assess which indoor housing environments offer fewer constraints and better movement opportunities. Full article