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Authors = Amadeo Sena-Torralba ORCID = 0000-0003-1340-1383

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14 pages, 2198 KiB  
Article
Lateral Flow Microimmunoassay (LFµIA) for the Reliable Quantification of Allergen Traces in Food Consumables
by Amadeo Sena-Torralba, Javier Gabaldón-Atienza, Aitor Cubells-Gómez, Patricia Casino, Ángel Maquieira and Sergi Morais
Biosensors 2022, 12(11), 980; https://doi.org/10.3390/bios12110980 - 7 Nov 2022
Cited by 12 | Viewed by 7681
Abstract
Quality assurance and food safety are of great concern within the food industry because of unknown quantities of allergens often present in food. Therefore, there is an ongoing need to develop rapid, sensitive, and easy to use methods that serve as an alternative [...] Read more.
Quality assurance and food safety are of great concern within the food industry because of unknown quantities of allergens often present in food. Therefore, there is an ongoing need to develop rapid, sensitive, and easy to use methods that serve as an alternative to mass spectrometry and enzyme-linked immunosorbent assay (ELISA) for monitoring food safety. Lateral flow immunoassay is one of the most used point-of-need devices for clinical, environmental, and food safety applications. Compared to traditional methods, it appears to be a simple and fast alternative for detecting food allergens. However, its reliability is frequently questioned due to the lack of quantitative information. In this study, a lateral flow microimmunoassay (LFµIA) is presented that integrates up to 36 spots in microarray format in a single strip, providing semi-quantitative information about the level of allergens, positive and negative controls, internal calibration, and hook effect. The LFµIA has been evaluated for the on-site simultaneous and reliable quantification of almond and peanut allergens as a proof of concept, demonstrating high sensitivity (185 and 229 µg/kg, respectively), selectivity (77%), and accuracy (RSD 5–25%) when analyzing commercial allergen-suspicious food consumables. Full article
(This article belongs to the Special Issue Biosensing Systems for Allergen and Allergy Testing)
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